HLA-DRB1基因多态性与HBV宫内感染的相关性

目的 探讨HLA-DRBl等位基因多态性与HBV宫内感染的关系.方法 选择HBsAg阳性孕妇分娩的新生儿中发生HBV宫内感染的母亲21例(A组),而未发生宫内感染的母亲46例(B组)以及正常母亲42例(C组),采用聚合酶链反应-序列特异性引物(PCR-SSP)技术扩增HLA-DRB1四对等位基因,计算各刑别出现频率.结果 (1)HLA-DRB1*11在A组的等位基因频率明显高于B组(38.1% vs 13.0%)(P<0.05);(2)HLA-DRB1*13、HLA-DRB1*03在A、B两组的等位基因频率明显低于C组(4.5% vs 33.3%,9.0%vs 28.6 0A)(P<0.01):(3)HLA-DRB1*07等位基因频率在A、B、C三组间差异无显著性.结论 HLA-DRB1*11与HBV宫内感染有关;HLA-DRB1*03、13可能与HBV清除有关.

The association between polymorphism of HLA-DRB1 antigens and intrauterine infection of HBV

Objective To investigate the association between the polymorphism of human leukocyte antigen(HLA)-DRB1 and the susceptibility to intrauterine infection of hepatitis B virus (HBV). Methods HBsAg positive pregnant women (67 cases) were divided into intrauterine infection (group A, 21 cases),non-intrauterine infection (group B,46 cases). Four genes were detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) in groups of A, B and C (42 normal subjects). Results The gene frequency of HLA-DRB1 * 11 in group A was significantly higher than that in group B (P<0.05). The gene frequencies of HLA-DRB1 * 03,13 in groups of A and B were significantly higher than those in group C (4.5% vs 33.3%, 9.0% vs 28.6%) (P<0. 01). There was no statistical difference of the gene frequency of HLA-DRB1 * 07 among three groups. Conclusion HLA-DRB1 * 11 is closely related to intrauterine infection of HBV, while HLA-DRB1 * 03,13 is connected with the cleaning up of HBV.