| 神经生长因子诱导神经干细胞向胆碱能神经元的分化 |
| |
| 引用本文： | 刘佳梅,陈东,孟晓婷.神经生长因子诱导神经干细胞向胆碱能神经元的分化[J].神经解剖学杂志,2005,21(6):603-606. |
| |
| 作者姓名： | 刘佳梅 陈东 孟晓婷 |
| |
| 作者单位： | 1. 吉林大学基础医学院,组织学与胚胎学教研室,长春,130021
2. 广东医学院,组织学与胚胎学教研室,湛江,524023 |
| |
| 基金项目： | 教育部高等学校博士学科点专项科研基金（20030183048）资助项目 |
| |
| 摘 要： | 为了探讨神经生长因子(NGF)是否具有诱导神经干细胞(NSCs)向胆碱能神经元分化的能力,利用无血清培养技术从胎鼠脑内获得神经干细胞,传代纯化后利用免疫细胞化学技术,观察不同剂量NGF作用后神经干细胞向胆碱乙酰转移酶(ChAT)阳性细胞分化的情况。结果发现:50、100、200ng/ml NGF组ChAT阳性细胞数明显比对照组增加,且以100ng/ml组最为明显;各NGF组,分化的细胞状态较好,且突起明显比对照组增粗增长,以200ng/ml组最为明显。此结果证明NGF具有诱导NSCs向胆碱能神经元分化的趋势,且能促进分化细胞突起的生长。
|
| 关 键 词： | 神经干细胞 神经生长因子 胆碱能神经元 |
| 收稿时间： | 2005-03-29 |
| 修稿时间： | 2005年3月29日 |
STUDY ON NEURAL STEM CELLS DIFFERENTIATED INTO CHOLINERGIC NEURON INDUCED BY NGF |
| |
| Liu Jiamei,Chen Dong,Meng Xiaoting.STUDY ON NEURAL STEM CELLS DIFFERENTIATED INTO CHOLINERGIC NEURON INDUCED BY NGF[J].Chinese Journal of Neuroanatomy,2005,21(6):603-606. |
| |
| Authors: | Liu Jiamei Chen Dong Meng Xiaoting |
| |
| Abstract: | In order to study the abihtv of NSCs differentiation into cholinergie neuron induced by NGF. NSCs were obtained by serum-free medium technique. The expression of ChAT was detected by immunocytochemistry. The results showed that the number of ChAT positive cells were significantly increased in 50, 100, 200 ng/ml groups compared with control group. The length of total primary neurite in 50, 100, 200 ng/ml NGF groups were obviously longer than that in control group. These results suggest that NSCs could differentiate into cholinergic neuron in vitro induced by NGF. |
| |
| Keywords: | neural stem cells cholinergic neuron NGF |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
