糖尿病大鼠脑缺血再灌后神经元损伤与细胞死亡

目的:研究脑缺血/再灌后迟发性神经元损伤的细胞死亡形式,以及在糖尿病作为附加因素时,对于神经细胞死亡的影响。方法:在糖尿病模型及中脑动脉阻塞(MCAO)/再灌模型的基础上,通过病理形态,FCM,TUNEL,琼脂糖凝胶电泳等检测手段对脑缺血及再灌后脑细胞死亡形式进行综合判定。结果:局灶脑缺血再灌损伤过程中既存在细胞坏死,又存在细胞凋亡。其凋亡的神经细胞主要分布在重度缺血区域的边缘(半影区)附近。缺血中心区主要表现为坏死。另外还证明了合并糖尿病组可明显加重缺血性脑损伤,而且与诱导半影区、中线区的细胞凋亡发生发展有密切关系。结论:局灶脑缺血/再灌后神经元损伤包括坏死与凋亡,中心区以前者为主,而半影区则以后者为主,二者具有紧密的内在联系。合并有糖尿病时,可以明显加重缺血再灌性脑损伤的进程。

Neuronal damage and cell death following ischemia/reperfusion in diabetic rats

AIM: To study forms of cell death following cerebral ischemia/reperfusion in diabetic rats. METHODS: Based on the modles of diabetes and middle cerebral artery occlusion(MCAO), characteristics of cell death after ischemia/reperfusion were evaluated synthetically by the pathological, flow cytometry(FCM), TUNEL and the DNA agarose electrophoresis.RESULTS: The occurrence of cerebral injury after ischemia/reperfusion were accompanied by cell necrosis and cell apoptosis. And cell apoptosis was mainly located in ischeamic penumbra(IP) zone around the densely ischemic focus. Ischemic centre(IC)was characterized by cell necrosis. At the same time, the results showed that the process of ischemic cerebral injury worsen by diabetes mellitus was related to inducing cell apoptosis in IP and Mid zone.CONCLUSION: Neuronal damage following focal cerebral ischemia/reperfusion included cell necrosis and apoptosis, IC zone was mainly characterized by the former, however IP zone by the latter, and there had close internal relationship between them. Brain damage following cerebral ischemia/reperfusion was worsen instinctly under diabetic condition.