| The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively |
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| 作者姓名： | Jingxian Yang Shuyuan Li Guozhu Han Yuan Lin |
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| 作者单位： | DepartmentofPharmacology,DalianMedicalUniversity,Dalian116027,China |
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| 基金项目： | ThisworkwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina(No.30070203) |
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| 摘 要： | Objective: To reveal the special feature of calponin (CaP) on myosins of different states. Methods: Myosin phosphorylafion determination, myosin Mg^2 -ATPase measurement and protein binding assay were used in this study， The lowest CaP/myosin ratio used in the assay was 1/lO000(mol/mol), which was 10 thousands-fold lower than the CaP/myosin ratio used in previous studies. Results: In the absence of actin, micro-amount of calponin (MAC) stimulated the Mg^2 -ATPase activities of myosin in different states slightly but significantly; and more importantly, MAC significantly increased the precipitations of unphosphorylated myosin, Ca^2 -dependently and independently phosphorylated myosins by MLCK but not the myosin phosphorylated by PKA. Conclusion: MAC has a high efficient and selective effect on myosin in the absence of acfin.
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| 关 键 词： | 钙结合蛋白 肌浆球蛋白 磷酸化 MLCK PKA 肌动蛋白 |
The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively |
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| Jingxian Yang,Shuyuan Li,Guozhu Han,Yuan Lin.The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively[J].Acta Universitatis Medicinalis Nanjing,2005,19(3):111-115. |
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| Authors: | Jingxian Yang Shuyuan Li Guozhu Han Yuan Lin |
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| Institution: | 1. Department of Pharmacology, Dalian Medical University, Dalian 116027, China
2. Radiology of department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective: To reveal the special feature of calponin (CaP) on myosins of different states. Methods: Myosin phosphorylation determination, myosin Mg~(2+)-ATPase measurement and protein binding assay were used in this study. The lowest CaP/myosin ratio used in the assay was 1/10000(mol/mol), which was 10 thousands-fold lower than the CaP/myosin ratio used in previous studies. Results: In the absence of actin, micro-amount of calponin (MAC) stimulated the Mg~(2+)-ATPase activities of myosin in different states slightly but significantly; and more importantly, MAC significantly increased the precipitations of unphosphorylated myosin, Ca~(2+)-dependently and independently phosphorylated myosins by MLCK but not the myosin phosphorylated by PKA. Conclusion: MAC has a high efficient and selective effect on myosin in the absence of actin. |
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| Keywords: | micro-amount of calponin myosin phosphorylation myosin Mg~(2+)-ATPase activity precipitation |
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