ATP hydrolytic activity of an iron-stimulated P-type ATPase of mouse liver microsomes |
| |
Authors: | Takeda K Soeda S Arai M Kawamura M |
| |
Institution: | Division of Molecular Cell Biology, School of Medicine, University of Occupational and Environmental Health, Japan, Yahatanishi-ku, Kitakyushu 807-8555, Japan. |
| |
Abstract: | Mouse liver microsomes treated with octylthioglucoside were examined for iron-stimulated ATPase activity. The activity was about 6 mumol Pi/mg protein/hr under optimal conditions 300 mM KC1, 3 mM MgSO4, 10 mM glutathione(GSH), 100 microM FeCl3, 3 mM ATP and 50 mM acetate buffer at pH 5.0]. The Km for iron was 20 microM. A reducing agent, such as GSH or dithiothreitol, was required for the activity, and removal of Fe2+ from the reaction mixture by bathophenan-throlinedisulfonate resulted in a complete loss of the iron-stimulated ATPase activity. Vanadate inhibited the iron-stimulated ATPase activity. These results suggest that microsomes from mouse liver contain the Fe(2+)-stimulated P-type ATPase. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|