缺血预处理对离体大鼠心脏肌钙蛋白T释放的影响

目的 :探讨缺血预处理对离体大鼠再灌注期释放心肌肌钙蛋白 T(Tn T)的影响。方法 :实验采用L angendorff灌流装置 ,单次 5 m in缺血 ,继 10 min再灌注作为预处理模型。于 2 0 min缺血后 6 0 min再灌注 ,测定冠流液Tn T、磷酸肌酸激酶 (CK)、乳酸脱氢酶 (L DH)和左室内压 (L VP)。结果 :缺血预处理能明显减少 Tn T、CK、L DH的释放 ,也能改善左室心肌收缩功能。∑Tn T或再灌注 6 0 m in时的 Tn T与 L VP有明显相关性。结论 :Tn T可以作为一个有效的生化指标来判断左室功能障碍和心肌受损程度 ,缺血预处理能减轻心肌缺所致的心肌损伤并改善左室功能

Effects of isthemis preconditioning on the release of cardiac troponin T in isolated hearts

The aim of this study was to examine the effect of ischemic preconditioning(IP) on the releases of cardiac troponin T(TnT) during reprefusion in isolated rat hearts.The hearts were perfused on a langendorff apparatus,IP was investigated with 5 min ischemic,followed by 10 min of reperfusion.After 20 min of globa ischemic,the releases of TnT,creatine kinase (CK),and lactate dehyodrogenase (LDH)in coronary effluent and the left ventricular developed pressure(LVP)were measured during 60 min of reperfusion,The results showed IP sinificantly reduced the release of TnT during reperfusion,and also improved contractile dysfunction.There were good inverse relationships between the LVP and the total amounts of TnT released during reperfusion period(∑TnT)or TnT levels at 60 min of reperfusion.Conclusion:cardiac TnT can be used a useful biochemical marker for the degree of myocardial cell damage and the function of left cardiac ventricle.IP can decrease the ischemin-induced myocardial injury.