抗氧化酶对隐睾生精细胞凋亡的影响

目的 :探讨超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSHPx)对隐睾生精细胞凋亡的影响。　方法 :4 8只未成熟SD雄性大鼠随机分为隐睾组和对照组 ,于术后第 1、3、7d采集睾丸。TUNEL法检测其生精细胞凋亡 ;化学比色法测定其SOD、CAT、GSHPx的活性和丙二醛 (MDA)含量。　结果 :术后第 7d ,与对照组相比 ,隐睾重量、SOD活性、CAT活性和SOD/ (CAT +GSHPx)比值均显著降低 (P均 <0 .0 1) ,GSHPx的活性无显著变化 (P >0 .0 5 ) ,生精细胞凋亡指数和MDA含量均显著增加 (P均 <0 .0 1)。　结论 :隐睾生精细胞的凋亡与抗氧化酶活性的降低密切相关

The Effect of Antioxidant Enzyme on Germ Cell Apoptosis in Cryptorchidism

Objectives: To evaluate the effect of superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSHPx) on germ cell apoptosis in cryptorchidism. Methods: Forty eight immature male Sprague Dawley rats were divided into the cryptorchid and the control group randomly.Testes were harvested at 1,3 and 7 days after the operation. Germ cell apoptosis was determined by TUNEL technique, at the same time the activities of antioxidant enzymes and the level of malonic diethyl aldehyde (MDA) were detected with spectrophotometer. Results:At the 7th day, as compared with the control, testicular weight, SOD activities, CAT activities and the ratio of SOD/(CAT GSHPx)were reduced significantly( P <0.01), GSHPx activities had no apparent change( P >0.05), while apoptosis index and the MDA level increased predominatly in cryptorchidism( P < 0.01 ). Conclusions: Germ cell apoptosis in cryptorchidism related closely to the decrease of antioxidant enzyme activity.