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| 重组人血管内皮生长因子(hVEGF121)工程菌的发酵及表达产物的分离纯化 | |
| 引用本文： | 周红霞,周梅仙,廖忠平,吴洁,胡卓逸,刘景晶. 重组人血管内皮生长因子(hVEGF121)工程菌的发酵及表达产物的分离纯化[J]. 药物生物技术, 2006, 13(1): 9-13 |
| 作者姓名： | 周红霞 周梅仙 廖忠平 吴洁 胡卓逸 刘景晶 |
| 作者单位： | 1. 中国药科大学生命科学与技术学院微基因药物实验室,江苏,南京,210009;南京晓庄学院生命科学系,江苏,南京,210017 2. 南京晓庄学院生命科学系,江苏,南京,210017 3. 中国药科大学生命科学与技术学院微基因药物实验室,江苏,南京,210009 |
| 摘 要： | 该研究优化了重组人血管内皮生长因子hVEGF121工程菌的发酵条件，考察乳糖诱导浓度和时间对目的蛋白表达量的影响。经乳糖诱导，目的蛋白以包涵体形式存在。通过菌体裂解、包涵体洗涤、裂解等初步纯化后，再经过DEAECellulose离子交换柱纯化和复性，经SDS-PAGE分析显示得到电泳纯的hVEGF121。 |
| 关 键 词： | 血管内皮生长因子 表达 纯化 包涵体 |
| 文章编号： | 1005-8915（2006）01-0009-05 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005-07-112005-10-25 |
Isolation and Purification of Recombinant Human Vascular Endothelial Growth Factor (hVEGF) Expressed in E.coli |
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| ZHOU Hong-xia,ZHOU Mei-xian,LIAO Zhong-ping,WU Jie,HU Zhuo-yi,LIU Jing-jing. Isolation and Purification of Recombinant Human Vascular Endothelial Growth Factor (hVEGF) Expressed in E.coli[J]. Pharmaceutical Biotechnology, 2006, 13(1): 9-13 | |
| Authors: | ZHOU Hong-xia ZHOU Mei-xian LIAO Zhong-ping WU Jie HU Zhuo-yi LIU Jing-jing |
| Affiliation: | 1. Department of Bioengineering, China Pharmaceutical University, Nanjing 210019, China; 2. Department of Biology, Nanjing Xiaozhuang College, Nanjing, 210017, China |
| Abstract: | The expression condition of recombinant human vascular endothelial growth factor 121 in E. coli expression system is optimized in this paper. After being induced by 2% lactose for 6h, recombinant protein was expressed. Results showed that gene highly expressed its product in inclusion body. By denaturation, purification with DEAE-cellulose chromatography and renaturation, the inclusion body was purified. The purity of product was above 90% analyzed by SDS-PAGE. It can be concluded that the recombinant human VEGF121 was expressed and purified successfully. |
| Keywords: | VEGF Expression Purification Inclusion Body |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |