Antiangiogenic properties of fibstatin, an extracellular FGF-2-binding polypeptide |
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Authors: | Bossard Carine Van den Berghe Loic Laurell Henrik Castano Caroline Cerutti Martine Prats Anne-Catherine Prats Hervé |
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Affiliation: | Institut National de la Santé et de la Recherche Médicale (INSERM) U589, C.H.U. Rangueil, Toulouse, France. |
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Abstract: | By using the two-hybrid system with basic fibroblast growth factor (FGF-2) as bait, we isolated and characterized fibstatin, an endogenous M(r) 29,000 human basement membrane-derived inhibitor of angiogenesis and tumor growth. Fibstatin, a fragment containing the type III domains 12-14 of fibronectin, was produced as a recombinant protein and was shown to inhibit the proliferation, migration, and differentiation of endothelial cells in vitro. Antiangiogenic activity of fibstatin was confirmed in a Matrigel angiogenesis assay in vivo, and electrotransfer of the fibstatin gene into muscle tissue resulted in reduced B16F10 tumor growth. Taken together, these results suggest that fibstatin could act as a powerful molecule for antiangiogenic therapy. |
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