主要促进因子超家族成员MFSD2A对肝癌细胞增殖、凋亡和侵袭的影响

目的 探索主要促进因子超家族蛋白2A (major facilitator superfamily domain containing 2A,MFSD2A)对肝癌细胞增殖、凋亡和侵袭的影响。方法 使用慢病毒载体构建差异表达MFSD2A的肝癌细胞株HepG2和SMMC-7721,由qPCR及Western blotting法评估其转染效率;CCK-8法分析差异表达MFSD2A对细胞增殖的影响,流式细胞术测定转染后细胞的基础凋亡率的变化,Transwell小室细胞侵袭实验明确MFSD2A对细胞侵袭能力的影响。结果 在肝癌细胞中上调MFSD2A表达可以显著抑制细胞增殖、促进基础凋亡,同时还可降低细胞的侵袭能力;而下调MFSD2A则可导致相反的效应。结论 MFSD2A在肝癌的肿瘤进程中发挥抑癌基因的功能,这种功能主要是通过抑制细胞增殖并诱导凋亡实现的。

Effects of major facilitator superfamily domain containing 2A on the cell proliferation,apoptosis and invasion of human hepatocellular carcinoma

Objective To explore the effects of major facilitator superfamily domain containing 2A (MFSD2A) on cell proliferation, apoptosis and invasion of hepatocellular carcinoma. Methods Hepatocellular cancer cell lines HepG2 and SMMC-7721 were transfected with lentivirus vector carried shRNA to knock down MFSD2A expression, meanwhile they were also transfected with lentivirus vector carried full length MFSD2A sequence to overexpress MFSD2A level. The qPCR and Western blotting were applied to evaluate the transfection efficiency. CCK-8 assay was used to investigate the functional effect of MFSD2A on cell proliferation. The cell apoptosis was detected with flow cytometry. The Transwell invasion assay was applied to determine the cell invasion and migration of the transfected cells. Results Upregulation of MFSD2A in hepatocellular cancer cells could significantly suppress the cell proliferation, induce the apoptosis, and decrease the cell invasion. On the contrary, downregulation of MFSD2A led to the opposite effect. Conclusion MFSD2A acted as a novel tumor suppressor gene for hepatocellular carcinoma through cell proliferation inhibition and apoptosis induction.