电针对帕金森病模型小鼠纹状体背侧的c-Fos表达的选择性调节

目的 观察电针对帕金森病(Parkinson’s disease, PD)模型小鼠纹状体内原癌基因蛋白(c-Fos)表达的选择性调控作用,以探究电针治疗PD的潜在机制。方法 制备6-羟基多巴胺(6-hydroxydopamine, 6-OHDA)右侧损伤的PD模型小鼠,实验分为对照组、对照+电针组、模型组和模型+电针组。电针施加于小鼠下肢双侧的“足三里”和“三阴交”穴位,采用100 Hz电针刺激持续30 min,继而用免疫组化检测纹状体背侧、腹侧和扣带回皮质的c-Fos蛋白表达。结果 模型组小鼠右侧背部纹状体c-Fos表达较对照组显著降低(P<0.001),而电针刺激显著增加了模型组右侧背部和腹部纹状体的c-Fos的表达(P<0.05)。检测扣带回皮质的c-Fos表达,各组之间差异无统计学意义,电针未产生明显的调控作用。结论 电针可区域特异性地激活PD小鼠损伤侧纹状体背部神经元的活性。

Electroacupuncture selectively regulates the expression of c-Fos in the dorsal striatum of Parkinsonian mice

Objective To observe the selective regulation of electroacupuncture (EA) on the expression of proto oncogene protein (c-Fos) in the striatum of Parkinsonian mice, so as to explore the potential mechanism of EA in the treatment of Parkinson's disease (PD). Methods PD model mice with right side injury by 6-hydroxydopamine (6-OHDA) were made. The mice were divided into Sham, Sham+EA, Model and Model + EA groups. The “Zusanli” and “Sanyinjiao” acupoints on both sides of the lower limbs of mice were used as the stimulation sites. 100 Hz EA was applied for 30 minutes, and the c-Fos protein expression in the dorsal and ventral striatum and cingulate cortex were detected by immunohistochemistry later. Results Compared with the Sham group, the expression of c-Fos in the right dorsal side of the Model mice was significantly lower (P<0.001), and EA stimulation increased the expression of c-Fos in the right dorsal and ventral side of the Model group (P<0.05). In the cingulate cortex, there was no significant difference in the expression of c-Fos among these groups, and EA had no obvious regulatory effect. Conclusion EA can specifically activate the activity of neurons in the dorsal striatum on the injured side of parkinsonian mice.