通山甜茶对大鼠血脂及抗氧化功能的影响

目的研究通山甜茶对大鼠的血脂及抗氧化能力的影响。方法雄性Wistar大鼠50只，实验前检测各组大鼠血中TC含量，按照TC的含量进行排序，然后进行分层抽样到5个不同组：正常对照组，高脂模型对照组，低剂量甜茶＋高脂饲料组，中剂量甜茶＋高脂饲料组，高剂量甜茶＋高脂饲料组。正常对照组大鼠进食普通饲料，高脂模型对照组大鼠进食高脂饲料，其余3组大鼠在进食高脂饲料的同时分别给予低、中、高剂量的通山甜茶。28d后取血检测TC、TG、HDL-C值，取大鼠的肝脏检测丙二醛（MDA）的含量、超氧化物岐化酶（S0D）活性和谷胱甘肽过氧化物酶（GSH-Px）活性。结果与模型对照组相比，高剂量甜茶组TC、TG含量显著下降（P〈0.05）；各剂量组对高密度脂蛋白胆固醇均无明显升高作用；低、中、高剂量组大鼠肝脏中MDA的含量均显著性降低（P〈0．01）；中、高剂量组大鼠肝脏中S0D的活性显著升高（P〈0．01）；高剂量组大鼠肝脏中GSH-Px活性显著升高。结论通山甜茶可降低实验性大鼠的血脂含量，并能增强大鼠的抗氧化能力。

Influence of Tongshan sweet tea on serum lipid level and antioxidation in rats

Objective To study the influence of Tongshan sweet tea in different doses on serum lipid and antioxidative capacities in rats. Methods Fifty male rats were divided into five groups: normal group, model control group, low dose of sweet tea plus fat rich food, medium dose of sweet tea plus fat rich food; high dose of sweet tea plus fat rich food. The level of serum lipid and Malondialdehyde (MDA), the activities of Superoxide Dismutase (SOD)and Glutathione Peroxidase (GSH-Px) in liver were determined on 29 days. Results Compared with model control group, the level of TC and TG in high dose group decreased (P< 0.05 ); the level of HDL-C had no difference in all groups; the content of MDA in different dose groups significantly decreased(P<0.01), the activities of SOD in medium dose and high dose group evidently increased(P<0.01) and the activities of GSH-Px in high group also reduced(P<0.01).Conclusion Tongshan sweet tea can decrease the level of serum lipid and enhance the antioxidative ability in rats.