Rolipram对THP-1巨噬细胞源性泡沫细胞胆固醇流出和ABCA1表达的影响

目的　以THP 1巨噬细胞源性泡沫细胞为研究对象 ,探讨磷酸二酯酶抑制剂rolipram对THP 1巨噬细胞源性泡沫细胞ABCA1表达及细胞内胆固醇流出的影响。方法　用液体闪烁计数器检测细胞内胆固醇流出 ,运用逆转录 -多聚酶链反应和Westernblot印迹分别检测ABCA1mRNA与ABCA1蛋白的表达 ,采用低pH值EIA法测定细胞内cAMP水平。结果　实验显示PDE4抑制剂rolipram能引起THP 1巨噬细胞源性泡沫细胞cAMP水平、ABCA1表达和apoA 1介导的胆固醇流出成平衡增加 ,而细胞内总胆固醇、游离胆固醇与胆固醇酯明显减少。结论　PDE4抑制剂rolipram增加THP 1巨噬细胞源性泡沫细胞ABCA1表达及细胞内胆固醇流出 ,这为防治动脉粥样硬化发生发展提供一个新的策略

Effect of rolipram on ATP binding cassette transporter 1 and cholesterol efflux in THP-1 macrophage-derived foam cell

AIM To study the effect of rolipram on ATP binding cassette transporter 1 and cholesterol efflux in THP 1 macrophage derived foam cell. METHODS After exposure of the cultured THP 1 macrophage derived foam cell to rolipram for different time, cholesterol efflux and ABCA1 mRNA and protein level were determined by FJ 2107P type liquid scintillator and reverse trancriptase polymerase chaim reaction(RT PCR) and western blot, respectively. RESULTS Rolipram promoted cholesterol efflux in THP 1 macrophage derived foam cell with time; RT PCR and western blot showed that exposure of the cultured THP 1 macrophage derived foam cell to rolipram for different time, resulted in increase in the expression of ABCA1 mRNA and protein in THP 1 macrophage derived foam cell, respectively; High performance liquid chromatography and enzyme immunoassay showed that exposure of the cultured THP 1 macrophage derived foam cell to rolipram for different time, resulted in decreasing Cholesterol and cholesterol ester in THP 1 macrophage derived foam cell with time and increasing cAMP level in THP 1 macrophage derived foam cell with time. CONCLUSION Rolipram promotes cholesterol efflux and increases the expression of ABCA1 in THP 1 macrophage derived foam cell. cAMP phosphodiesterase 4 inhibitors may provide a novel strategy for treatment of atherosclerosis.