Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro |
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Authors: | Head David J Lee Zoe E Swallah Muhammed M Avent Neil D |
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Affiliation: | Genomics Research Institute and Centre for Research in Biomedicine, University of the West of England, Frenchay, Bristol, UK. |
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Abstract: | CD47 ligation has been shown to induce phosphatidylserine (PS) expression as part of a death pathway in nucleated blood cells. Using Annexin V binding assays we showed that ligation of CD47 with the specific CD47-binding peptide 41NK, anti-CD47 monoclonal antibody, and its natural ligand thrombospondin-1 also induced PS expression on enucleated erythrocytes. PS expression was associated with a concomitant loss of erythrocyte viability in vitro. Further characterisation of the CD47-PS signalling pathway on erythrocytes may help develop clinical strategies to further preserve the life of blood donations and improve our understanding of certain types of haemolytic anaemias. |
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Keywords: | CD47 erythrocytes phosphatidylserine thrombospondin-1 viability |
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