Feasibility of Using a Near-Infrared Device for the Determination of Nicotine in Cigarette Smoke Presented to Animals in Inhalation Studies

Abstract

Summary: Nicotine in smoke presented to animals in inhalation studies is currently determined by placing glass-fiber pads (“Cambridge“ pads) in unoccupied animal ports in the smoking machine and pulling a fixed volume of smoke through the pad. The nicotine is extracted from the pad and quantified using gas chromatography (GC). A near-infrared (NIR) instrument was used to develop an alternative technique, which provides estimates of nicotine concentrations on Cambridge pads in only 1–2 min. For given cigarette types, the NIR data are highly correlated with the GC data. This new method results in considerable time-saving during inhalation studies with cigarette smoke.