Class-II and IL 2 receptor positive cells in the pancreas of NOD mice |
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| Authors: | A Signore A Cooke P Pozzilli G Butcher E Simpson P C L Beverley |
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| Institution: | (1) ICRF, HTIG, Faculty of Clinical Sciences, University College London, London, UK;(2) Department of Immunology, The Middlesex Hospital, Medical School, London, UK;(3) Department of Diabetes and Immunogenetics, St. Bartholomew's Hospital, London, UK;(4) Cambridge Research Station, AFRC, Institute of Animal Physiology and Genetic Research, Babraham Hall, Cambridge, UK;(5) Transplantation Biology Center, Clinical Research Center, Harrow, Middlesex, UK;(6) Cattedra Endocrinologia (I), Clinica Medica (II), University of Rome  La Sapienza , Rome, Italy |
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| Abstract: | Summary The aberrant expression of Class-II molecules on pancreatic B cells in Type 1 (insulin-dependent) diabetes is still a matter of debate. In order to verify if Class-II molecules are expressed on islet cells in the NOD mouse we have studied 21 female mice of different ages (5 to 22 weeks). Serial cryostat pancreas sections were stained with monoclonal rat antibodies against Class-II antigens (P7/7) and the IL2 receptor (AMT-13). Our results show no Class-II expression by endocrine cells at any age, whereas about 25–32% of mononuclear cells infiltrating the islets were Class-II positive, and only 6–9% were IL2 receptor positive. No staining, except of occasional tissue macrophages, was observed in the pancreas of BALB/c, CBA or B10.SCSN mice. Our data are in contrast with those recently published and therefore the reality of expression of Class-II molecules by islet cells of NOD mice should be viewed with caution. |
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| Keywords: | Type 1 (insulin-dependent) diabetes Class-II molecules IL2 receptor NOD mice insulitis activated lymphocytes lymphocyte subsets |
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