端粒重复扩增-微孔板杂交法检测妇科肿瘤端粒酶活性的研究

目的：探讨妇科几种常见恶性肿瘤与端粒酶活性的关系。方法：采用端粒重复扩增（TRAP）扩增端粒酶产物，通过生物素－亲和素将扩增产物结合于微孔板，并与荧光素（FITC）标记的特异性探针杂交，经酶标抗荧光素抗体结合面显色。结果：妇科恶性肿瘤端粒酶活性检出25例（89．3％），而肿瘤边缘组织检出14例（50．0％），正常对照组织检出10例（35．7％）。恶性肿瘤端粒酶活性显著高于肿瘤远端组织和正常对照组织（P＜0．05）。结论：端粒酶激活可能在妇科恶性肿瘤的发生、发展中起重要作用。TRAP－微孔板杂交法是检测端粒酶活性灵敏而特异的一种方法。

STUDY OF THE TELOMERASE ACTIVITY IN GYNECOLOGIC CARCINOMAS USING TELOMERE REPEAT AMPLIFICATION-MICROWELL PLATE HYBRIDIZATION

Objective:To investingate the relationship between telomerase activities and some common gynecologic carcinomas.Method:Telomerase activities were measured by the telomere repeated amplification protocol(TRAP)-microwell plate hybridization system.Result:Telomerase activities in gynecologic carcinomas were found by 89.3% (25/28),in the margin tissues between carcinoma and normal 50% (14/28) and in normal tissues only 35.7%(10/28).Telomerase activities in gynecologic carcinomas were significantly higher than in the margin and normal tissues(P<0 05).Conclusion:Activation of telomerase may play an important role in gynecologic carcinomas.TRAP-microwell plate hybridization system is a specific and sensitive assay to detect the telomerase activity.