动态检测特异性免疫复合物在脑囊虫病治疗过程的意义

目的　了解特异性免疫复合物在脑囊虫病治疗过程中的意义。方法　用双单抗夹心ELISA法检测特异性免疫复合物 :采用抗人IgG、IgM单抗包板 ,来捕获由血清提取的免疫复合物中的特异性IgG、IgM型免疫复合物 ,通过酶标抗囊虫单抗结合物显色 ,测其OD值。结果　IgG型免疫复合物在 1疗程后含量较治疗前明显升高 (P <0 .0 5 )以后各疗程则显著下降 (P <0 .0 1) ;IgM型免疫复合物的含量治疗前与 1疗程后相比差别不显著 ,但随着疗程增加其含量逐渐下降 (P <0 .0 1) ;各疗程的临床反应加重发生率以第 1疗程最高 (P <0 .0 0 5 )。结论　IgG型免疫复合物可以作为治疗反应、疗效考核的依据

DYNAMIC MONITORING OF THE SPECIFIC IMMUNE COMPLEX DURING TREATMENT IN NEUROCYSTICERCOSIS

Aim To observe the dynamic changes of specific immune complex over all courses of drug administration of patients with neurocysticercosis. Methods By using a double mono-cloned antibody sandwich ELISA assay the specific immune complex was detected.Employed anti-human IgG, IgM McAb as coated material, and HRP labeled anti-cysticerci McAb as recognizing system, specific immune complexes that extracted in sera were monitored. Results The immune complex level of IgG class rose significantly after the 1st course (P<0.05), and then gradually dropped off (P<0.01). On the other hand, that of IgM class did not change significantly after 1st course of treatment. It gradually declined with advancing course of drug administration (P<0.01). The incidence rate of clinical worsening after 1st course of treatment was the highest one among all courses (P<0.005).Conclusion This study indicates that immune complex level of IgG class is associated with the clinical worsening and the efficacy of therapy.