| Inhibition of germ tube‘s formation of Candida albicans by lactobacillus spp. |
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| 引用本文： | 唐欢,魏泓. Inhibition of germ tube‘s formation of Candida albicans by lactobacillus spp.[J]. 中国人民解放军军医大学学报, 2006, 21(5) |
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| 作者姓名： | 唐欢 魏泓 |
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| 作者单位： | Department of Animal Science College of Basic Medicine，Third Military Medical University，Department of Animal Science，College of Basic Medicine，Third Military Medical University，Chongqing 400038，China，Chongqing 400038，China |
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| 摘 要： | Objective:To investigate the effects of 6 strains of lactobacilli and their main culture metabolites on the morphogenesis of Candida albicans (C. albicans) so as to screen medicinal strains as antifungal agents. Methods: Spent culture supernatant (SC'S) at different culture time points, live lactobacilli, heat-killed bacteria and bacterial short-chain fatty acids (SCFA) with different molarities were incubated with C. albicans respectively. Crystal violet-based germ tube assay was used to detect the germination of C. al-bicans and the inhibitory efficiency of each addition was tested by germination rate. Results: SCS of lacto-bacillus spp. decreased the germination significantly and live bacteria of L. rhamnosus. GG, L. acidophilus 1.050103-12 as well as L. johnsonii JCM1022 could partially inhibit the conversion, however, all the heat-killed bacteria failed to control the germ tube formation. In addition, only butyric acid blocked the conversion of yeast to hypha among all the SCFA. Conclusion: The excellent fungistasis activities make L. rhamnosus. GG. L. acidophilus L050103 12 and L. johnsonii JCM1022 potential strains as antifungal drugs and the inhibition seems to have direct correlation to the metabolites.
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Inhibition of germ tube''''s formation of Candida albicans by lactobacillus spp. |
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| TANG Huan,WEI Hong. Inhibition of germ tube''''s formation of Candida albicans by lactobacillus spp.[J]. Journal of Medical Colleges of PLA(China), 2006, 21(5) |
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| Authors: | TANG Huan WEI Hong |
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| Affiliation: | Department of Animal Science,College of Basic Medicine,Third Military Medical University,Chongqing,400038,China Department of Animal Science,College of Basic Medicine,Third Military Medical University,Chongqing,400038,China |
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| Abstract: | Objective:To investigate the effects of 6 strains of lactobacilli and their main culture metabolites on the morphogenesis of Candida albicans (C. albicans) so as to screen medicinal strains as antifungal agents. Methods: Spent culture supernatant (SCS) at different culture time points, live lactobacilli, heatkilled bacteria and bacterial short-chain fatty acids (SCFA) with different molarities were incubated with C. albicans respectively. Crystal violet-based germ tube assay was used to detect the germination of C. albicans and the inhibitory efficiency of each addition was tested by germination rate. Results: SCS of lactobacillus spp. decreased the germination significantly and live bacteria of L. rhamnosus. GG, L. acidophilus L050103-12 as well as L. johnsonii JCM1022 could partially inhibit the conversion, however, all the heatkilled bacteria failed to control the germ tube formation. In addition, only butyric acid blocked the conversion of yeast to hypha among all the SCFA. Conclusion: The excellent fungistasis activities make L.rhamnosus. GG, L. acidophilus L050103-12 and L. johnsonii JCM1022 potential strains as antifungal drugs and the inhibition seems to have direct correlation to the metabolites. |
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| Keywords: | lactobacillus short-chain fatty acids germ tube candidiasis antifungal agent |
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