流式细胞术检测肾上腺肿瘤DNA含量及临床意义

目的 明确ＤＮＡ量测定是否枳和为肾上腺肿瘤良、恶性诊断的指标之一。方法 采用ＰＣＭ技术对１３例肾肯朱嗜铬细胞列肾上腺皮质肿瘤，肾上腺转移瘤和肉眼正常肾上腺组织各５例新鲜标本进行了ＤＮＡ含量测定。结果 病理为肾上腺嗜铬细胞瘤及其瘤旁肾上腺标本ＤＮＡ含量多为非整倍体（１２／１３），但求后无１例复发和转移，而６全 理为皮质腺瘤标本２例ＤＮＡ含量为非整倍体，其中１例术后出现肝转移而确诊为恶性，１例为二倍体

Nuclear DNA content in adrenal tumor by flow cytometry and its clinical significance

Objective To determine the diagnostic value of detecting nuclear DNA content by flow cytometry in adrenal tumor.Methods DNA ploidy was investigated in fresh samples from 13 cases of pheochromocytomas,7 adrenocortical tumors,5 secondary adrenal carcinoma and 5 normal adrenal tissue.Results The results showed that samples from 12 pheochromocytomas and normal adrenal glands adjunct to pheochromocytomas were aneuploid.Among 6 adrnocortical adenomas,2 cases were aneuploid,one of which died from liver metastasis,while 1 case carcinoma was diploid,who was alive without evidence of disease.Conclusion The assessment of ploidy level by flow cytometry is useful in the diagnosis of adrenocortical neoplasms,but as for pheochromocytoma,it is less useful.