疏肝健脾方对乳腺增生病大鼠血清性激素及ER、PR表达的影响

目的 通过观察中药疏肝健脾方对乳腺增生病大鼠乳腺组织形态和血清性激素及其受体的影响,探讨疏肝健脾方防治乳腺增生病的机制.方法 选用SPF级雌性Wistar大鼠60只,随机分为空白对照组、疾病模型组、三苯氧胺组、小剂量药物组、大剂量药物组,采用苯甲酸雌二醇和黄体酮建立乳腺增生动物模型,分别灌服三苯氧胺、大小剂量疏肝健脾方煎液,观察大鼠乳腺组织形态和血清性激素及其受体的变化.结果 疏肝健脾方能够可使乳腺小叶腺泡数明显减少、腺泡腔缩小及腺导管扩张程度减轻,降低乳腺增生大鼠血清雌二醇(E2)、垂体促乳素(PRL)含量,升高孕激素(P)、睾酮(T)含量,部分升高FSH和LH的含量.明显抑制ER、PR的阳性表达强度.结论 疏肝健脾方可通过调节乳腺增生大鼠的性激素水平,降低实验大鼠乳腺组织中ER、PR含量,使其对雌激素的敏感性下降,对大鼠乳腺增生有明显的抑制作用.

Effects of liver-smoothing and Spleen-srengthening Formula on Serum Sex Hormone and Expression of Estrogen Receptors (ER) and Progesterone Receptors (PR) of Rats with Hyperplasia of Mammary Gland.

Objective To investigate the prevention and treatment mechanism of liver-smoothing and spleen-srengthening formula on hyperplasia of mammary gland,we study the effects of it on morphology,histology,serum sex hormone and its receptor of rats with hyperplasia of mammary gland.Methods Sixty female Wistar rats(SPF)were randomized into 5 groups:control group,disease model group,the tamoxifen group,small dose of medicine group and large-dose drug group.Estradiol Benzoate and Progesterone were used to establish an...