| 湘南地区泌尿生殖道感染沙眼衣原体的基因型分布 |
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| 引用本文： | 张小清,戴国知,李建萍,左克强,张国卿.湘南地区泌尿生殖道感染沙眼衣原体的基因型分布[J].中国民康医学,2006,18(19):829-830. |
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| 作者姓名： | 张小清 戴国知 李建萍 左克强 张国卿 |
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| 作者单位： | 1. 湘南学院基础医学课部,湖南,郴州,423000
2. 郴州市第一人民医院检验科 |
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| 基金项目： | 湖南省教育厅科研项目（04C610）;郴州市科技局科研项目（项目号：05CK56-1）. |
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| 摘 要： | 目的：了解湘南地区泌尿生殖道感染沙眼衣原体分离株的基因型（血清型）分布情况。方法：应用聚合酶链反应和限制性片段长度多态性分析，对湘南部分地区泌尿生殖道感染沙眼衣原体病人的62份阳性标本进行了基因分型。结果：沙眼衣原体基因型中，E型25份，D型14份，F型9份，G型7份，J型4份；有3份不典型基因型，未进一步分型。结论：湘南地区泌尿生殖道沙眼衣原体感染的基因型别以E型为主，其次为D型、F型、G型、J型。
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| 关 键 词： | 沙眼衣原体 基因分型 |
| 收稿时间： | 09 4 2006 12:00AM |
Distribution of Genotype of Chlamydiia trachomatic from the Cervical and urethral swabs in Southern Hunan Area |
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| ZHANG Xiao - qing , DAI Guo - zhi, LI Jian - ping, et al.Distribution of Genotype of Chlamydiia trachomatic from the Cervical and urethral swabs in Southern Hunan Area[J].medical journal of chinese peoples health,2006,18(19):829-830. |
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| Authors: | ZHANG Xiao - qing DAI Guo - zhi LI Jian - ping |
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| Abstract: | Objective:To investigate the distribution of Chlamydia trachomatis serovars from detected cases of Chlamydia trachomatis infection in partial cities in Southern Hunan Area.Methods:The major outer membrane protein gene of Chlamydia trachomatis was amplified by nested polymerase chain reaction(nPCR) from clinical specimens collected in Chenzhou city.Restriction fragmemt length polymorphism(RFLP) were analyzed after Alu I and Msp I,digestion.Results: Sixty-two specimens were amplified and genotyped by RFLP.The most prevalent genotype was serotype E(25),followed by D(14),F(9),G(7),J(4) and others(3)untypical.Conclusions: The distribution of Chlamydia trachomatis serovars in Southern Hunan Area was that the most prevalent genotype was serotype E,followed by D,F,G and J. |
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| Keywords: | Chlamydia trachomatis restriction fragment length polymorphism genotype |
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