CXCR7在SDF-1介导内皮细胞参与血管形成中的作用

目的探讨CXCR7在SDF-1诱导内皮细胞参与血管生成中的作用。方法用Western blot和流式细胞仪检测脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)中CXCR7和CXCR4的表达;利用小分子拮抗剂分别阻断CXCR4或CXCR7,然后通过MTT、Transwell小室法及三维基质胶血管样结构形成实验分别考察CXCR7和CXCR4对SDF-1诱导HUVECs增殖、迁移及管样结构形成能力的影响。结果 HUVECs细胞中同时高表达CXCR4和CXCR7;CXCR7的拮抗剂显著抑制SDF-1诱导HUVECs的增殖(P<0.01);而SDF-1诱导HUVECs迁移却被CXCR4的拮抗剂阻止(P<0.01);CXCR7和CXCR4的拮抗剂均显著阻止SDF-1诱导HUVECs形成血管样结构(P<0.01)。结论 CXCR7和CXCR4在SDF-1诱导HUVECs参与血管生成的过程中均起着不可或缺却又不尽一致的作用,SDF-1诱导HUVECs的增殖主要通过CXCR7发挥作用,而CXCR4主要参与SDF-1诱导HUVECs的迁移,两者共同参与SDF-1诱导HUVECs形成管样结构的调节。

Role of CXCR7 in angiogenesis involving SDF-1-mediated endothelial cells

Objective To study the role of CXCR7 in angiogenesis involving SDF-1-mediated endothelial cells.Methods Expression of CXCR7 and CXCR4 in human umbilical vein endothelial cells(HUVEC) was detected by Western blotting and flow cytometry,respectively.CXCR4 or CXCR7 was blocked with small molecule antagonists.Effect of CXCR4 and CXCR7 on proliferation,migration,and tube-like structure formation of SDF-1-mediated HUVEC was tested by MTT,Transwell chamber,3D-gel tube formation assay,respectively.Results CXCR4 and CXCR7 were expressed in HUVEC.The proliferation of HUVEC induced by SDF-1 was significantly inhibited by CXCR7 antagonist(P<0.01);The migration of HUVEC induced by SDF-1 was inhibited by CXCR4 antagonist(P<0.01);While the tube-like structure formation of HUVEC was inhibited by either blocking of CXCR4 or CXCR7(P<0.01).Conclusion CXCR7 and CXCR4 play an important but a different role in angiogenesis involving SDF-1-mediated HUVEC.SDF-1-mediated HUVEC proliferate via CXCR7 while CXCR4 is mainly involved in the migration of SDF-1-mediated HUVEC.Both CXCR7 and CXCR4 participate in the tube-like structure formation of SDF-1-mediated HUVEC.