| 高效液相色谱法测定复方银黄胶囊中黄芩苷含量 |
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| 引用本文： | 李永斌,谭志艺,李依信.高效液相色谱法测定复方银黄胶囊中黄芩苷含量[J].中国现代药物应用,2010,4(5):4-5. |
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| 作者姓名： | 李永斌 谭志艺 李依信 |
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| 作者单位： | 广东省云浮市药品检验所,527300 |
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| 摘 要： | 目的建立高效液相色谱法测定复方银黄胶囊中黄芩苷的含量。方法色谱柱为Diamonsil（钻石）C18（250×4．6mm，5μ），流动相为甲醇-水-冰醋酸（50：50：1），检测波长为280nm，流速为1．0ml／min，柱温为30℃。结果黄芩苷进样量在0．0997～1．994μg范围内与峰面积有良好的线性关系，r=0．9999；平均加样回收率为99．82％，RSD=1．13％（n=6）。结论该方法简便、灵敏、结果准确、重复性好，可用于复方银黄胶囊的质量控制。
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| 关 键 词： | 复方银黄胶囊 黄芩苷 高效液相色谱法 含量测定 |
Determination of Baicalin Contents in Compound Capsules of Yinghuang by HPLC |
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| LI Yong-bin,Tan Zhi-yi,LI Yi-xin.Determination of Baicalin Contents in Compound Capsules of Yinghuang by HPLC[J].Chinese Journal of Modern Drug Application,2010,4(5):4-5. |
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| Authors: | LI Yong-bin Tan Zhi-yi LI Yi-xin |
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| Institution: | .( Yunfu Institute for Drug Control of Guangdong, Yunfu,527300, China) |
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| Abstract: | Objective To establish a HPLC method for determination of Baicalin Contents in Compound Capsules of Ying huang. Methods The column was Diamonsil Cl8 （ 250 × 4. 6 mm, 5 μ）. The mobile phase was methanol -wateracetic acid （50： 50：1 ）. The flow rate was 1.0 ml/min, and the UV detective wavelength was set at 280 nm. The column temperature was 30℃. Results The liner range of the calibration curve of Baicalin was 0. 0997 - 1. 994μg with a correlation coefficient of 0. 9999. The average recovery rate was 99. 82%, RSD = 1.13 % （n = 6）. Conclusion The method is simple, high sensitivity and accurate with good repeatability and can be used for quality control in Compound capsules of Yinghuang. |
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| Keywords: | Compound capsules of Yinghuang Baicalin HPLC Content determination |
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