Translocation chromosome 7 of A431 cells contains amplification and rearrangement of EGF receptor gene responsible for production of variant mRNA |
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Authors: | John H Hunts Nobuyoshi Shimizu Tadashi Yamamoto Kumao Toyoshima Glenn T Merlino Young-hua Xu Ira Pastan |
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Institution: | (1) Department of Molecular and Cellular Biology, University of Arizona, 85721 Tucson, Arizona;(2) Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160 Tokyo, Japan;(3) Institute of Medical Science, University of Tokyo, 4-6-1 Shiroganedai, Minato-Ku, 108 Tokyo, Japan;(4) Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis, National Cancer Institute, NIH, 20205 Bethesda, Maryland |
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Abstract: | The epidermal growth factor (EGF) receptor, along with several oncogene protein products, possesses tyrosine-specific protein kinase activity. Furthermore, the EGF receptor has structural similarity to the putitive v-erb-B transforming protein. Because of these closely shared characteristics, it is important to elucidate the possible involvement of the EGF receptor in malignant transformation. The epidermal carcinoma cell line A431 exhibits an abnormally high number of EGF receptors, which is associated with the presence of translocation chromosome M4. Recently, A431 cells have been shown to contain amplified sequences for the EGF receptor gene(s) and also to produce a variant mRNA which diverges from the normal EGF receptor mRNA at the 3 end. Here we report, using the human EGF receptor cDNA probe pE7, that the chromosome M4 has a six-to sevenfold amplification of the EGF receptor gene. Furthermore, the presence of M4 in somatic cell hybrids correlates with the production of the variant 2.9-kb mRNA. This aberrant mRNA is apparently generated by an intrachromosomal rearrangement which was detected using as a probe a fragment of the pE15cDNA encoding the variant mRNA. |
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