针刺对VD大鼠记忆障碍及c—fos mRNA和蛋白表达的影响

目的：探讨针刺对血管性痴呆大鼠（VD）学习记忆障碍的改善及其与c-fos的关系。方法：采用4－血管阻断全脑缺血再灌注制备VD大鼠模型，针刺脑、肾耳穴后，作原位分子杂交、免疫组化、行为学检测、图像分析，结果：治疗后VD大鼠海马CA1区c-fos mRNA和蛋白表达与学习记忆成绩呈正相关。结论：耳针改善VD大鼠学习记忆，可能与针刺加强c-fos的表达，保护缺血后海马神经元的作用有关。

Effects of acupuncture on deficit of memory function and expression of c - fos mRNA and protein in vascular dementia rats

Objective:To study the relationship between auricular acupuncture improve deficit of memory function and expression of c-fos in hippocampus CA1 region in vascular dementia rats. Method: The vascular dementia model of rat was performed by 4-vessel occlusion method. Two auricular acupuncture points of the kidney and the brain were chosed to treat VD rats. The rats were tested by Y-type maze and c-fos mRNA and protein were examined in hippocampus CA1 region of rat by using in situ hybridization, immunohistochemistry and computer image analysis. Result: The expression of c-fos mRNA and protein showed increase in hippocampus CA1 region of rat after auricular acupuncture treatment, which had a significant positive correlation with memory. Conclusion: The auricular acupuncture could improve learning and memory capability of VD rats. The protective effect of auricular acupuncture on hippocampus neurons in VD may be related to the increase of c-fos.