9~12岁超重肥胖男童性激素与脂代谢谱研究

目的探索9~12岁超重肥胖男孩性激素、脂质代谢的特异性变化和相互关系。方法 72名9~12岁男童被分为正常体重组(n=42)、超重组(n=15)和肥胖组(n=15)。应用血液激素检测和基于超高效液相色谱-四极杆飞行时间质谱(UPLC/QTOF/MS)的非靶向脂质组学方法,对72名青春期前体重异常男孩的性激素水平和脂代谢谱检测,利用多元统计分析比较不同肥胖程度男孩性激素水平和脂代谢谱的差异。结果 (1)超重组和肥胖组的血清性激素结合球蛋白(SHBG)含量显著低于正常体重组,且肥胖组显著低于超重组(P<0.05);超重组和肥胖组的血清脱氢表雄酮(DHEA)含量显著高于正常体重组(P<0.05)。(2)非靶向脂质组学方法鉴定并筛选出超重组与正常体重组组间脂质差异物171种,肥胖组与正常体重组组间脂质差异物218种,超重组与肥胖组组间脂质差异物34种,其中超重&肥胖组与正常体重组均有的差异脂质物有150种。(3)SHGB与脂质差异物磷脂酰胆碱(PC)(20∶0/0∶0)和PC[18∶1(9E)/0∶0]呈正相关(r=0.6,P<0.05),DHEA与脂质差异物甘油二酯(DG)[17∶2(9Z,12Z)/22∶0/0∶0]呈正相关(r=0.5,P<0.05)。结论 9~12岁超重肥胖男童存在骨龄提前现象且与DHEA水平的升高有关,但性激素水平与体格发育不同步;超重肥胖男童血清DHEA显著升高的同时,DG[17∶2(9Z,12Z)/22∶0/0∶0]水平明显上调,血清SHGB显著降低的同时,大多数PC显著下降,其作用机制有待进一步研究。

Study on the relationship between sex hormone and lipid metabolism in overweight and obese boys aged 9 to 12 years

Objective To explore the specific changes and correlations of sex hormones and lipid metabolism in overweight and obese boys aged 9-12 years.Methods Seventy-two male subjects(9 to 12 years old)were divided into normal weight group(n=42),overweight group(n=15),and obese group(n=15).Plasma sex hormone levels and lipid levels were detected by chemiluminescence immunoassay and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC/QTOF/MS).All data were analyzed by multivariate statistics analysis.Results(1)The serum concentration of sex hormone-binding globulin(SHBG)in obese group and overweight group was significantly lower than that in normal weight group,and the serum concentration of SHBG in obese group was significantly lower than that in overweight group(P<0.05).The serum concentrations of dehydroepiandrosterone(DHEA)in overweight and obese group were significantly higher than those in normal weight group(P<0.05).(2)There were 171 kinds of differential lipid compounds between normal weight group and overweight group,218 species of differential lipid compounds between normal weight group and obese group,and 34 species of differential lipid compounds between overweight and obese group.Among them,150 kinds of lipid compounds in normal weight group were significantly different from those in overweight and obese group.(3)SHGB was positively correlated with phosphatidyl cholines(PC)(20∶0/0∶0)and PC[18∶1(9E)/0∶0](r=0.6,P<0.05),DHEA was positively correlated with diacylglycerol(DG)[17∶2(9Z,12Z)/22∶0/0∶0](r=0.5,P<0.05).Conclusions 9-12 years old overweight and obese boys had early growth and development,but sex hormone levels were not synchronized with growth and development.The serum DHEA level in overweight and obese boys was significantly increased,while the level of DG[17∶2(9Z,12Z)/22∶0/0∶0]was significantly increased,and the serum SHGB concentration was significantly decreased,while the vast majority of PC was significantly down-regulated.The mechanism of these changes needs to be further studied.