液相色谱-原子荧光联用测定作业场所空气中的无机砷

目的 建立液相色谱-原子荧光光谱法测量作业场所空气中亚砷酸盐As(Ⅲ)与砷酸盐As(V)含量的方法.方法 样品经0.15 mol/L硝酸90 ℃浸提1h后,以15 mmol/L磷酸氢二铵为流动相,CNWSep AX(250 mm × 4.0 mm× 10 μm)(LAEQ-4025G7)色谱柱分离,分析检测空气样品中A...

Determination of airborne inorganic arsenic in workplaces by liquid chromatography-atomic fluorescence

Objective To establish a method for determination of As (Ⅲ) and As (Ⅴ) in the air of workplaces by liquid chromatography-atomic fluorescence spectrometry. Methods The samples were extracted by 0.15 mol/L HNO3 solution at90 ℃ for 1 h,then As (Ⅲ) and As(V) were separated and analyzed with the mobile phase of 15 mmol/L diammonium hydrogen phosphate,using CNWSep AX(250 mm × 4.0 mm × 10 μm)(LAEQ-4025 G7) column. Results In the range of0-100 g/L,the linear correlation coefficients(r) of As (Ⅲ) and As(V)were 0.999 9 and 0.999 8,respectively. The detection limits of As (Ⅲ) and As(V) were 0.14 g/L and 0.38 g/L,and the elution efficiencies were 96.77%-98.66%,96.77%-98.66% respectively. The recoveries of As (Ⅲ) and As(V) were 90.67%-95.60%,91.82%-95.84%,and the relative standard deviations were 0.47%-1.44% and 0.48%-1.34% respectively. Conclusions This pretreatment method can avoid morphological changes of inorganic arsenic species. The method has good separation effect on two inorganic arsenic species,and has high sensitivity and accuracy,and is suitable for the determination of inorganic arsenic in the air of workplaces.