百日咳蛋白对小鼠过敏性哮喘模型的免疫调节作用

目的：观察百日咳杆菌蛋白对致敏小鼠气道炎症、气道高反应性以及IFN-γ／IL-4平衡的调节作用。方法：致敏小鼠，卵白蛋白反复攻击后静脉注射乙酰甲胆碱（Mch），测定气道反应性；收集支气管肺灌洗液（BALF），检测炎症细胞及IL-4、IFN—γ水平，进行肺组织病理学检查。结果：肌注或滴鼻给予百日咳杆菌蛋白，能抑制致敏小鼠肺阻力及肺动态顺应性变化，上调肺泡灌洗液中IFN-γ／IL-4比例，降低嗜酸性粒细胞聚集，并且作用呈现剂量依赖性。病理学检查显示，百日咳杆菌蛋白能够有效抑制致敏小鼠气道上皮杯状细胞增生及肺组织中炎症细胞浸润。结论：百日咳杆菌蛋白能有效抑制过敏性哮喘小鼠肺部炎症、改善肺功能，对防治哮喘具有潜在的应用前景。

Immunoregulatory effects of pertussis protein on allergic asthma in mouse

OBJECTIVE: To investigate the immunoregulatory effects of pertussis protein on airway inflammatory, IFN-gamma/IL-4 ratio in bronchoalveolar lavage fluids(BALF) and airway hyperresponsiveness (AHR) in the sensitized mice. METHODS: The sensitized mice were reexposed to ovalbumin and the airway response to methacholine injection was monitored. Inflammatory cells and cytokines IFN-gamma/IL-4 ratio in BALF were measured. Lung tissue specimens were collected for histological examination. RESULT: Intramuscular injection or intranasal instillation of pertussis protein inhibited changes in lung resistance and lung dynamic compliance, upregulated IFN-gamma/IL-4 ratio and decreased eosinophil accumulation in a dose-dependent manner. Pathological examination showed that goblet cell hyperplasia and inflammatory cells infiltration in lung tissue were suppressed by pertussis protein. Conclusion: Pertussis protein inhibits the inflammation and regulates the function of lungs in asthma mice, suggesting its potential application in treatment of asthma.