| HBsAg高浓度值血清稀释液及稀释比例的选择 |
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| 引用本文： | 孙文萱,张学东,马莉.HBsAg高浓度值血清稀释液及稀释比例的选择[J].职业与健康,2010,26(13):1488-1489. |
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| 作者姓名： | 孙文萱 张学东 马莉 |
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| 作者单位： | 1. 河南省新乡市中心医院,453000
2. 河南省郑州安图绿科生物工程有限公司 |
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| 摘 要： | 目的探讨乙肝病毒表面抗原(HBsAg)高浓度标本的准确稀释方法。方法采用化学发光法定量检测HBsAg。从体检和临床标本中筛选出77份大于180ng/ml的HBsAg高浓度值血清进行梯度稀释,然后进行定量检测。结果 4种稀释液中添加牛血清蛋白(BSA)最为合适,4种稀释液对于这种高值血清的最适稀释比例为1∶100和1∶10000。结论对于HBsAg高浓度标本,采取合适的稀释液,按照一定比例稀释后再进行定量检测,能够得到HBsAg准确结果 。
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| 关 键 词： | 表面抗原 高浓度 稀释液 稀释比例 |
Selection of HBsAg Serum Dilution of High Concentration and Dilution Ratio |
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| SUN Wen-xuan,ZHANG Xue-dong,MA Li.Selection of HBsAg Serum Dilution of High Concentration and Dilution Ratio[J].Occupation and Health,2010,26(13):1488-1489. |
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| Authors: | SUN Wen-xuan ZHANG Xue-dong MA Li |
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| Institution: | ( Xinxiang Central Hospital,Henan,450016,China) |
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| Abstract: | Objective]To investigate the accurate dilution method for high concentration samples of HBsAg.Methods]Chemoluminescence method ？was adopted for quantitative detection of HBsAg. A total of 77 serum samples of HBsAg high concentration （180 ng/ml） screened from physical examination and clinical specimens were conducted gradient dilution,and then were quantitatively detected.Results]BSA was appropriately added in 4 kinds of dilution,and the optimal dilution ratio of high concentration serum was 1：100 and 1：10 000.Conclusion]For high concentration HBsAg serum samples,quantitative determination after appropriate dilution with a certain proportion can get an accurate result of HBsAg. |
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| Keywords: | Surface antigen high concentration dilution dilution ratio |
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