非促分裂型haFGF对H2O2损伤心肌细胞的保护作用研究

目的：了解活性氧自由基对心肌细胞的损伤状况，并在此基础上探讨非促分裂型haFGF(nm-haFGF)对心肌细胞的保护作用。方法： 采用胰蛋白酶消化法，体外分离和培养新生SD乳鼠心肌细胞，建立H2O2损伤心肌细胞的模型，检测细胞存活率、细胞凋亡和观察细胞形态学的变化，以评价H2O2对心肌细胞的损伤状况，并加入一定浓度的nm-haFGF，观察心肌细胞的存活率、SOD活性和MDA含量的改变，以及凋亡的变化。结果： H2O2对心肌细胞的损伤呈浓度依赖地加剧；在H2O2压力作用下，10-80 μg/L nm-haFGF逐步提高H2O2损伤心肌细胞的存活率，其中80 μg/L组的细胞存活力明显大于对照组(P＜0.05)，同时逐步提高SOD活性和降低MDA含量，其中40和80 μg/L组的SOD活性明显大于阳性对照组(P＜0.05)，40和80 μg/L组的MDA含量明显小于阳性对照组(P＜0.05)；此外，在0.01 mmol/L H2O2作用24 h后，10 μg/L和40 μg/L nm-haFGF组的心肌细胞凋亡百分率分别为32.4%和10.7%。结论： 心肌细胞的损伤与H2O2的浓度呈剂量依赖关系；nm-haFGF对由H2O2引起的心肌细胞损伤具有拮抗作用。

The cardiac protection of non-mitogenic human fibroblast growth factor on cardiomyocytes treated with hydrogen peroxide in vitro

AIM: To study the protective effects of non-mitogenic human fibroblast growth factor (nm-haFGF) on cardiomyocytes injured by reactive oxygen free radicals. METHODS: The cardiomyocytes were isolated from neonatal SD mouse by trypsin digestion. The cardiomyocytes injury model was established by exposing the cells to hydrogen peroxide (H2O2), and the injury status in cardiomyocytes were evaluated by examining the cellular viability, measuring cell apoptosis and observing the change of cellular morphology. nm-haFGF was added to the culture medium, and the changes of cellular viability, superoxide dismutase (SOD), malondialdehyde (MDA) and celluar apoptosis were observed. RESULTS: A dose-dependence relation between the concentration of H2O2 and the cardiomyocytes injury was observed. 10-80 μg/L nm-haFGF dose-dependently increased cardiomyocyte viability and the general SOD activity, as well as decreased the content of MDA and the quantity of cardiomyocyte apoptosis. CONCLUSION: The higher the concentration of H2O2, the more serious the cardiomyocyte injury. nm-haFGF may have a good protective effects on cardiomyocytes treated with H2O2.