采用组织工程方法体外构建血管模型的初步实验研究

目的　探索体外构建组织工程化人工血管的可行性。方法　通过酶消化法分离牛主动脉血管内皮细胞，培养、传代，纯化。采用交联胶原包埋处理聚羟基乙酸纤维无纺网(PGA)，将第3～7代血管内皮细胞接种于上述材料上，通过特制的旋转装置，缓慢动态旋转培养10天，使内皮细胞在管腔内表面附着生长，扫描电镜观察，采用6-酮-前列腺素-1α放射免疫测试药盒测定管形材料中内皮细胞释放前列环素量。结果　培养血管内皮细胞VIII因子相关抗原抗体染色呈阳性，内皮细胞在管腔内表面贴附良好，细胞之间融合成片，经10天培养，形成较完整内膜层，覆盖率为(91.2±1.5)%,前列环素生成率为（4.6±0.5）μg/cm

RECONSTRUCTION OF TISSUE ENGINEERED VASCULAR MODEL IN VITRO

OBJECTIVE: To explore the feasibility of reconstructing tissue engineered vessel in vitro. METHODS: Bovine endothelial cells were isolated from calf thoracic aorta by enzyme digestion methods and subcultured and purified. The endothelial cells of the 3rd to 7th passages were seeded into the inner surface of tubular scaffold material by polyglycolic acid(PGA) coated with cross-linked collagen, and cultured in vitro for 10 days using dynamic rotation culture technique. Scanning electron microscopy was used to analyse the morphological characteristics, and prostacyclin released by endothelial cells was measured by radioimmunoassay of 6-keto-prostaglandin F1 alpha. RESULTS: The VIII factor staining of cultured endothelial cells was positive. The endothelial cells adhered well on the inner surface of tubular scaffold material with confluent monolayer covering(91.2 +/- 1.5)%. The endothelialized model released prostacyclin at a rate of (4.6 +/- 0.5) micrograms/cm2.min. There was significant difference to control group (P < 0.05). CONCLUSION: The PGA coating with collagen is an ideal scaffold for endothelial cells, the coverage rate is increased through dynamic rotation culture technique. It will lay a good foundation for architecture of a laminated structure of tissue engineered vessel.