Fractionation of the water-soluble proteins from calf lens |
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| Authors: | M A Kibbelaar H Bloemendal |
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| Affiliation: | Department of Biochemistry, University of Nijmegen, Geert Grooteplein Noord 21, 6525 EZ Nijmegen, The Netherlands |
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| Abstract: | The water-insoluble proteins from calf lens have been subdivided into a urea-soluble (USL) and a urea-insoluble (UIL) part. USL can be fractionated into four components by gel filtration on Sephadex G 200 columns equilibrated in 6 m-urea. One of the fractions contains the major cytoskeletal proteins MP42 and MP55. The major fiber plasma membrane protein MP26 remains in the UIL fraction and can be isolated by preparative SDS-polyacrylamide gel electrophoresis. Peptide mapping reveals that MP26 is definitely different from the major β-crystallin component βBp. |
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