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HPLC法同时测定复方羟苯乙酯醇溶液中2组分的含量
引用本文:张军,李瑞林,赵尚清,宋蓓,陈新善,狄亚敏. HPLC法同时测定复方羟苯乙酯醇溶液中2组分的含量[J]. 中国药房, 2008, 19(25): 1979-1980
作者姓名:张军  李瑞林  赵尚清  宋蓓  陈新善  狄亚敏
作者单位:1. 武警北京总队医院药房,北京市,100027
2. 解放军总后勤部卫生部药品仪器检验所,北京市,100071
摘    要:目的:建立以高效液相色谱法同时测定复方羟苯乙酯醇溶液中羟苯乙酯及羟苯甲酯含量的方法。方法:色谱柱为Hypersil ODS,流动相为0.01mol·L-1磷酸二氢钾-甲醇-磷酸(50∶50∶0.4),检测波长为255nm,流速为8mL·min-1,进样量为20μL。结果:羟苯乙酯和羟苯甲酯检测浓度的线性范围分别为3.024~30.24(r=0.999 97)、1.022~10.22μg·mL-1(r=0.999 98);平均回收率分别为99.45%(RSD=0.48%)、99.67%(RSD=0.72%)。结论:本方法快速、准确、分离度好,可用于该制剂中2组分的含量测定。

关 键 词:羟苯乙酯  羟苯甲酯  高效液相色谱法  含量测定

Simultaneous Determination of Two Components in Compound Ethylparaben Alcoholic Solution by HPLC
ZHANG Jun,LI Rui-lin,ZHAO Shang-qing,SONG Pei,CHEN Xin-shan,DI Ya-min. Simultaneous Determination of Two Components in Compound Ethylparaben Alcoholic Solution by HPLC[J]. China Pharmacy, 2008, 19(25): 1979-1980
Authors:ZHANG Jun  LI Rui-lin  ZHAO Shang-qing  SONG Pei  CHEN Xin-shan  DI Ya-min
Affiliation:ZHANG Jun, LI Rui-lin, ZHAO Shang-qing, SONG Pei,CHEN Xin-shan, DI Ya-min(1.Dept. of Pharmacy, Beijing Corps Hospital, Chinese People's Armed Police Forces, Beijing 100027, China;2.Institute for Drug and Instrument Control of Health Department of General Logistics Department of PLA, Beijing 100071, China)
Abstract:OBJECTIVE: To establish an HPLC method for the simultaneous determination of the two components- ethylhydroxybenzoate(EH) and methylhydroxybenzoate(MH) in compound ethylparaben alcoholic solution. METHODS: The assay was conducted on a Hypersil ODS column with 0.01 mol ·L^-1 potassium dihydrogen phosphate - methanol - phosphoric acid (50 : 50 : 0.4) as mobile phase at a flow rave of 8 mL · min^-1. The detection wavelength was 255 nm and sample size was 20μL. RESULTS: The calibration curves were linear over the range from 3.024 to 30.24μg·mL^-1(r = 0.999 97) for EH, and from 1.022 to 10.22μg·mL^-1(r = 0.999 98) for MH. The average recoveries of EH and MH were 99.45% (RSD = 0.48% ) and 99.67% (RSD = 0.72% ), respectively. CONCLUSION: The HPLC method is simple, quick, accurate and well- separated, and it can be used for the simultaneously determination of MH and EH in compound ethylparaben alcoholic solution.
Keywords:Ethylhydroxybenzoate  Methylhydroxybenzoate  HPLC  Content determination
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