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正交试验优选淫羊藿总黄酮的提取工艺
引用本文:韩强,吕冬梅,王来成.正交试验优选淫羊藿总黄酮的提取工艺[J].中国药房,2008,19(30):2347-2348.
作者姓名:韩强  吕冬梅  王来成
作者单位:徐州医学院附属医院药剂科,徐州市,221002
摘    要:目的:优选淫羊藿总黄酮的提取工艺。方法:以淫羊藿总黄酮的含量为评价指标,以浸提温度、浸提时间、固液比和乙醇浓度为考察因素,采用正交试验优化淫羊藿总黄酮的提取工艺。结果:优化的提取条件为60%乙醇,固液比为1∶10,80℃回流提取2次,每次1h。结论:优化的提取工艺简便、合理,可为淫羊藿总黄酮工业化提取提供参考。

关 键 词:正交试验  淫羊藿  总黄酮  工艺优化

Optimization of the Extract Technology of the Total Flavonoids from Herba Epimedii by Orthogonal Experiment
HAN Qiang,LV Dong-mei,WANG Lai-cheng.Optimization of the Extract Technology of the Total Flavonoids from Herba Epimedii by Orthogonal Experiment[J].China Pharmacy,2008,19(30):2347-2348.
Authors:HAN Qiang  LV Dong-mei  WANG Lai-cheng
Institution:(Dept .of Pharmacy,The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China)
Abstract:OBJECTIVE:To optimize the extracting process of the total flavonoids from Herba Epimedii.METHODS: The extract technology of the total flavonoids from Herba Epimedii was optimized by orthogonal experiment with the yield of total flavonoids from Herba Epimedii as index and with the extraction temperature, extraction time, the ratio of solid to liquid and the concentration of ethanol as factors.RESULTS: The optimum extracting conditions for the total flavonoids from Herba Epimedii were as follows: the reflux extraction was conducted twice (1 hour/time) at 80℃ with 60% ethanol as solvent, and the ratio of solid to solution was 1 : 10.CONCLUSION: The optimum extracting technology is simple and reasonable, and it serves as a good reference for the extracting process of the total flavonoids from Herba Epimedii.
Keywords:Orthogonal experiment  Herba Epimedii  Total flavonoids  Technology optimization
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