外源性腺苷三磷酸和腺苷对食管癌TE-13细胞株凋亡的影响

目的　探讨腺苷三磷酸 (ATP)和腺苷 (ADO)是否具有诱导人食管低分化鳞癌细胞株TE 13凋亡的作用。方法　MTT法 ,AO/EB双荧光染色法 ,琼脂糖凝胶电泳 ,流式细胞仪方法。结果　ATP在0 .1～ 1.0mmol·L- 1或ADO在 0 .0 1～ 1mmol·L- 1浓度范围内 ,可不同程度地抑制TE 13细胞的增殖 ,ATP和ADO作用 72h后 ,对TE 13细胞的最大抑制率分别达 (80 .5± 6 .2 ) %和 (74 .0± 5 .3) %。细胞经0 .3mmol·L- 1的ATP或ADO处理 4 8h后 ,细胞形态出现了凋亡特征 ;电泳可见明显的“梯状”条带 ;ATP和ADO可浓度依赖性地诱导细胞的凋亡 ,1mmol·L- 1的ATP和ADO诱导细胞的凋亡率分别为 (16 .6± 1.1) %和 (16 .9± 1.2 ) %。结论　ATP和ADO均有诱导食管癌TE 13细胞凋亡的作用

Effects of extracellular adenosine triphosphate and adenosine on apoptosis of TE-13 esophageal carcinoma cells

AIM To study if adenosine triphosphate(ATP) and adenosine(ADO) could induce apoptosis of TE-13 human squamous esophageal carcinoma cells in vitro. METHODS MTT assay, AO/EB double staining, agar ose gel electrophoresis, flow cytometry. RESULTS ATP and ADO produced inhibitory effect on the proliferation of TE-13 cells at the concentration of 0.01－1.0 mmol&;#8226;L^-1. The maxium inhibition rate was （80.5±6.2）% or (74.0±5.3)%, respectively. The morphological changes of apoptosis and the ladder like pattern of DNA fragmentation were observed in a concentration-dependent manner. The maxium apoptotic rate of TE-13 at 48 h was（16.6±1.1）% or（16.9±1.2）%, respectively. CONCLUSION Both ATP and ADO can induce apoptotsis of TE-13 cell line.