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Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes
Authors:Laurent Poirel  Yu Larpin  Jan Dobias  Roger Stephan  Jean-Winoc Decousser  Jean-Yves Madec  Patrice Nordmann
Institution:1. Emerging Antibiotic Resistance, Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Fribourg, Switzerland;2. INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland;3. Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland;4. Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Zurich, Switzerland;5. Department of Virology, Bacteriology - Infection Control, Parasitology - Mycology, Assistance Publique - Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France;6. IAME, UMR 1137, INSERM, Paris, France;g. Agence Nationale de Sécurité Sanitaire (Anses), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France;h. Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
Abstract:The Rapid Polymyxin NP test has been recently developed to rapidly detect polymyxin resistance in Enterobacteriaceae. Here we evaluated this test for detecting MCR-1/MCR-2-producing Enterobacteriaceae using a collection of 70 non-redundant strains either recovered from the environment, animals, or humans. Sensitivity and specificity were found to be 100%.
Keywords:Polymyxin  MCR  Resistance  Enterobacteriaceae  Rapid Polymyxin NP test
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