2例CisAB/B亚型的血清学和分子生物学研究

目的 结合ABO血型血清学结果,采用ABO基因测序技术鉴定CisAB血型.探讨CisAB/B亚型的血清学和基因型的关系.方法 对于送至青岛市中心血站ABO血型待定的献血者标本,采用经典试管法进行血清学分析.采用聚合酶链反应(PCR)直接测序的方法分析ABO基因第6,7外显子及侧翼序列、启动子和增强子序列.对于有突变的标...

Serological and Genetic Characterization of CisAB/B Blood Sub-group: Analysis of Two Cases

Objective To identify the precise ABO blood type subgroup with gene sequencing and discuss the relationship between the serology and genotype in CisAB/B blood sub-group.Methods We screened ABO blood type of blood donors in the blood center.Samples were first examined with a routine serological method.Those with ambiguous results were further examined with genotyping to identify ABO subgroups.The exon 6,exon 7 and the flanking region of ABO gene as well as its promoter and enhancer region were sequenced by PCR.Mutations found in the PCR were further confirmed by haplotype sequencing method.Results Genotype of the two cases were both CisAB01/B101.Serological test showed the two cases to be A2B and AxB subgroup.Sequences of the promoter region and enhancer region of the two cases were the same.Conclusion It is diffi-cult to identify CisAB only by serological method.DNA sequencing combined with serological testing is able to precisely identify ABO blood subgroup.Individuals with the same genotype would show dif-ferent serological characteristics.