长链脂酰辅酶A合成酶1在肝癌中的表达及临床意义

目的探讨长链脂酰辅酶A合成酶1(Long-Chain Acyl-CoA Synthetase 1,ACSL1)在肝细胞癌(hepatocellular carcinoma,HCC)的表达及临床意义。方法收集334例诊断为HCC患者的癌组织与对应癌旁组织;免疫组化分析癌组织与癌旁组织中ACSL1的表达差异,并进一步分析HCC中ACSL1表达与患者临床病理特征的关系及对患者生存的影响;慢病毒转染构建ACSL1过表达的SNU739细胞;MTS、流式细胞术检测ACSL1对SNU739细胞的增殖和凋亡的影响;SeahorseXF24分析仪、乳酸试剂盒探究ACSL1对SNU739细胞能量代谢的影响。结果HCC组织中的ACSL1表达水平显著低于癌旁组织(3.02±2.57 vs 7.60±3.34,t=20.040,P=0.000);HCC中ACSL1的表达水平与患者的年龄有相关性(c2=16.472,P=0.000);ACSL1蛋白表达水平是HCC患者生存的危险因素(HR=1.642,P=0.012);ACSL1可以显著增强SNU739细胞凋亡(t=13.886,P=0.000)、线粒体基础氧耗速率(oxygen consumption rate,OCR)(F=83.429,P=0.001)以及线粒体最大氧耗率(F=859.792,P=0.000)。结论ACSL1在HCC组织中低表达,ACSL1低表达的HCC患者预后更差;ACSL1可作为HCC患者预后的潜在标志物,有助于筛查早期HCC,提高HCC患者生存率。

Expression and clinical significance of ACSL1 in hepatocellular carcinoma

Objective To explore the expression of long-chain acyl-CoA synthetase 1(ACSL1)in hepatocellular carcinoma(HCC)and its clinical values.Methods 334 paired HCC tissues were collected to detect the expression of ACSL1 by immunochemistry staining.The paper also analyzed the relationship between the expression of ACSL1 protein and the clinicopathological characteristics and prognosis of HCC patients.Lentivirus transfection was used to overexpression ACSL1 in HCC cell line SNU739.Cell proliferation and apoptosis were respectively measured by MTS assay and Flow cytometry.Cell energy metabolism were detected by SeahorseXF24 analyer and Lactate Assay Kit.Results The expression of ACSL1 in HCC tissues was markedly lower than that in adjacent tissues(3.02±2.57 vs 7.60±3.34,t=20.040,P=0.000),and the expression of ACSL1 was related to age of the patients(c2=16.472,P=0.000).The level of ACSL1 expression was a risk factor for survival in HCC patient(HR=1.642,P=0.012).ACSL1 was significantly promoted cell apoptosis(t=13.886,P=0.000),mitochondrial basal oxygen consumption rate(F=83.429,P=0.001)and maximal oxygen consumption rate(F=859.792,P=0.000)in SNU739 cell.Conclusion ACSL1 is low experssion in HCC tissues,and the prognosis of HCC patient with low ACSL1 expression is worse.ACSL1 can be used as a potential marker of prognosis in HCC patients,which is expected to be helpful to screen for early HCC and improve the survival rate of HCC patients.