| 流式细胞术检测HLA-B27两种试剂比较与结果分析 |
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| 引用本文： | 陈永强,郑璐,张玥,王杜平,程星,陈艳红,周忠海,牛国平. 流式细胞术检测HLA-B27两种试剂比较与结果分析[J]. 国际检验医学杂志, 2022, 43(2): 152-155. DOI: 10.3969/j.issn.1673-4130.2022.02.005 |
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| 作者姓名： | 陈永强 郑璐 张玥 王杜平 程星 陈艳红 周忠海 牛国平 |
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| 作者单位： | 徐州市中心医院检验科,江苏徐州 221009;徐州医科大学附属淮海医院实验科,江苏徐州 221004 |
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| 基金项目： | 江苏省双创博士项目(2020SC01);徐州市重点研发计划社会发展项目(KC20110);徐州市中心医院课题(2-2109024)。 |
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| 摘 要： | 目的 比较两种人类白细胞抗原B27(HLA-B27)检测试剂盒临床检测效果与异常结果 分析,为临床提供可靠的检测报告.方法 采用HLA-B27-FITC/HLA-B7-PE和HLA-B27-FITC/CD3-PE两种检测试剂盒检测1208例腰椎病、颈椎病、关节痛、脊柱僵硬和强直性脊柱炎患者标本中HLA-B27的表达,结...
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| 关 键 词： | 人类白细胞抗原B27 流式细胞术 强直性脊柱炎 |
Comparison and result analysis of two reagents for detecting HLA-B27 by flow cytometry |
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| CHEN Yongqiang,ZHENG Lu,ZHANG Yue,WANG Duping,CHENG Xing,CHEN Yanhong,ZHOU Zhonghai,NIU Guoping. Comparison and result analysis of two reagents for detecting HLA-B27 by flow cytometry[J]. International Journal of Laboratory Medicine, 2022, 43(2): 152-155. DOI: 10.3969/j.issn.1673-4130.2022.02.005 |
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| Authors: | CHEN Yongqiang ZHENG Lu ZHANG Yue WANG Duping CHENG Xing CHEN Yanhong ZHOU Zhonghai NIU Guoping |
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| Affiliation: | (Department of Clinical Laboratory,Xuzhou Central Hospital,Xuzhou,Jiangsu 221009,China;Department of Experiment,Huaihai Hospital Affiliated to Xuzhou Medical University,Xuzhou,Jiangsu 221004,China) |
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| Abstract: | Objective To compare the clinical detection effects and abnormal results analysis of two human leucocyte antigen(HLA)-B27 detection kits in order to provide a reliable laboratory report for clinical diagnosis.Methods A total of 1208 samples were tested by two reagents,including HLA-B27-FITC/HLA-B7-PE kit and HLA-B27-FITC/CD3-PE kit,and combined with clinical diagnosis,the results of the two methods were compared.Results Totally 12(0.99%)of 1208 patients detected by HLA-B27-FITC/HLA-B7-PE detection kit showed the percentage of HLA-B7+HLA-B27+cell population≥90%,and they were interpreted as HLA-B27 suspected positive,while HLA-B27-FITC/CD3-PE kit test results were all positive.In addition,unclear lymphocyte grouping was easy to cause HLA-B27-FITC/HLA-B7-PE kit to interpret HLA-B27 positive results as false negative,while the HLA-B27-FITC/CD3-PE kit could avoid false negative by using CD3 gate.Conclusion The results of HLA-B27 detection by the two detection kits are accurate and reliable,and each has its own characteristics. |
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| Keywords: | human leucocyte antigen-B27 flow cytometry ankylosing spondylitis |
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