miR-584下调CCN2对结直肠癌SW1116细胞增殖、侵袭的影响及机制研究

目的 探讨miR-584下调CCN2对结直肠癌SW1116细胞增殖、侵袭的影响及机制.方法 2020年1—4月于辽宁省肿瘤医院中心实验室进行实验,设立SW1116直肠癌细胞组、miR-NC组(空白载体)、miR-584 mimics组(过表达)、miR-584抑制剂组(低表达);各组细胞每孔设6个平行样,培养72 h....

Effect of miR-584 down regulating CCN2 on proliferation and invasion of colorectal cancer SW1116 cells and its mechanism

Objective To explore the effect and mechanism of miR-584 down-regulation of CCN2 on the proliferation and invasion of colorectal cancer SW1116 cells.Methods From January to April 2020,experiments were carried out in the Central Laboratory of Liaoning Cancer Hospital,setting up SW1116 rectal cancer cell group,miR NC group(blank vector),miR-584 mimics group(overexpression),and miR-584 inhibitor group(low expression).Each group of cells set up 6 parallel samples per well and cultured for 72 h.MTT method,Transwell method and flow cytometry were used to determine the levels of cell proliferation,invasion,and apoptosis.RT PCR and Western blotting were used to determine the mRNA and protein expression levels of miR-584 and CCN2.Results Compared with the SW1116 rectal cancer cell group,the miR-584 mimics group OD value,survival rate,number of single clones,number of transmembrane formation decreased,and apoptosis rate increased(P<0.05),miR-584 inhibitor group OD value,survival rate,single clone formation The number and the number of penetrating membrane increased,and the apoptosis rate decreased(P<0.05).Compared with the miR-584 mimics group,the miR-584 inhibitor group increased the OD value,survival rate,number of colonies formed,and number of membrane penetration,and decreased apoptosis rate(t=16.854,19.634,18.532,24.547,35.951,P=0.000).Compared with the SW1116 rectal cancer cell group,the miR-584 mRNA expression in the miR-584 mimics group was increased,and the CCN2 mRNA and protein expression was decreased(P<0.05).The miR-584 mRNA expression in the miR-584 inhibitor group was decreased,and the CCN2 mRNA and protein expression was increased(P<0.05).Compared with the miR-584 mimics group,the miR-584 mRNA expression in the miR-584 inhibitor group decreased,and the CCN2 mRNA and protein expression increased(t=28.654,26.254,19.965,all P=0.000).There was no statistically significant difference in the levels of the above indicators between the SW1116 rectal cancer cell group and the miR NC group(P>0.05).Conclusion miR-584 can inhibit the proliferation and invasion of colorectal cancer SW1116 cells,and promote their apoptosis.The mechanism may be related to the down-regulation of CCN2 expression by miR-584.