季节因素对男性精子受精功能的影响

目的 通过分析广东中山地区不同季节不孕门诊男性患者精子受精功能的变化,为自然受孕及辅助生殖治疗的最佳受精时机提供参考.方法 选取2019年3月至2021年5月中山市人民医院不孕门诊采集的精液标本7 109份作为研究对象,其中春季(3月至5月)2 772份,夏季(6月至8月)1 885份,秋季(9月至11月)1 579份...

Effects of seasonal factors on fertilization function of male sperm

Objective To provide reference for the best fertilization time of natural conception and assisted reproduction therapy, by analyzing the changes of sperm fertilization function of male infertility clinic patients in different seasons in Zhongshan of Guangdong province. Methods 7,109 semen samples were collected from Infertility Clinic of Zhongshan People′s Hospital from March 2019 to May 2021, including 2,772 semen samples in spring(March to May), 1,885 semen samples in summer(June to August), 1,579 semen samples in autumn(September to November), and 873 semen samples in winter(December to February). Routine semen analysis was performed on all semen samples, including 414 samples for sperm acrosomal enzyme quantitative determination, 275 samples for induced sperm acrosomal reaction determination, and 449 samples for sperm-hyaluronic acid binding determination. Results The number of PR grade sperm in summer (86.8±79.4)×10⁶] was significantly lower than that in spring (95.11±78.9)×10⁶], autumn (94.4±81.7)×10⁶] and winter (100.9±81.5) ×10⁶](P<0.05), but there was no statistical difference between autumn, winter and spring(P>0.05). Quantitative measurement of sperm acrosomal enzyme in spring (79.7±30.4)μIU/10⁶] was higher than that in summer (74.1±34.7)μIU/10⁶], autumn (72.4±33.9)μIU/10⁶] and winter (71.5±33.1)μIU/10⁶], with statistical difference(P<0.05), but there was no statistical difference between summer, autumn and winter(P>0.05). The sperm acrosomal response induced in spring (44.0±14.1)%] and winter (46.8±17.2)%] was higher than that in summer (38.1±11.6)%] and autumn (41.5±8.7)%], the differences were statistically significant(P<0.05), but there was no statistical difference between spring and winter and between summer and autumn(P>0.05). Sperm-hyaluronic acid binding assay in spring (57.3±12.3)%] and winter (57.4±12.2)%] was higher than that in summer (49.2±15.3)%] and autumn (51.8±14.5)%], the differences were statistically significant(P<0.05), but there was no statistical difference between spring and winter and between summer and autumn(P>0.05). Conclusions Seasonal factors have influence on semen routine parameters and fertilization function of infertility outpatients. In general, in spring and winter are good for clinical pregnancy, sperm fertilization function is better than in summer and autumn.