The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity |
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Authors: | Dydecka Aleksandra Bloch Sylwia Necel Agnieszka Topka Gracja Węgrzyn Alicja Tong Jinge Donaldson Logan W. Węgrzyn Grzegorz Nejman-Faleńczyk Bożena |
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Affiliation: | 1.Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland ;2.Department of Biology, York University, 4700 Keele Street, Toronto, ON, M3J 1P3, Canada ;3.Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, K?adki 24, 80-822, Gdańsk, Poland ; |
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Abstract: | The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein. |
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