| GAS5靶向miR-222-3p对牙周膜干细胞成骨分化的影响机制 |
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| 引用本文： | 郭烨,马庆云,赵文丽,段风.GAS5靶向miR-222-3p对牙周膜干细胞成骨分化的影响机制[J].实用口腔医学杂志,2021(2):271-274. |
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| 作者姓名： | 郭烨 马庆云 赵文丽 段风 |
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| 作者单位： | 北京市中关村医院口腔科;陕西中医药大学第二附属医院口腔科;西安医学院第三附属医院口腔科 |
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| 摘 要： | 成骨诱导hPDLSCs后,茜素红染色显示矿化增强GAS5表达升高(P<0.05),Runx2、ALP和OCN mRNA表达降低,miR-222-3p表达升高(P<0.05);miR-222-3p抑制剂可逆转GAS5低表达对hPDLSCs的作用(P<0.05)。提示hPDLSCs成骨分化后GAS5表达上调,下调GAS5可通过靶向调控miR-222-3p,抑制hPDLSCs成骨分化。
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| 关 键 词： | 牙周膜干细胞 GAS5 成骨分化 miR-222-3p |
The effects of miR-222-3p targeted by GAS5 on osteogenic differentiation of periodontal ligament stem cells |
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| GUO Ye,MA Qingyun,ZHAO Wenli,DUAN Feng.The effects of miR-222-3p targeted by GAS5 on osteogenic differentiation of periodontal ligament stem cells[J].Journal of Practical Stomatology,2021(2):271-274. |
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| Authors: | GUO Ye MA Qingyun ZHAO Wenli DUAN Feng |
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| Institution: | (Department of Stomatology,Beijing Zhongguancun Hospital,100190 China;Department of Stomatology,Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang;Department of Stomatology,Third Affiliated Hospital of Xi'an Medical College) |
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| Abstract: | Alizarin red staining showed that after osteogenesis induction the minerialization of human periodontal ligament stem cells(hPDLSCs)increased,GAS5 expression increased,the expression of Runx2,ALP and OCN mRNA decreased,and the expression of miR-222-3p increased(P<0.05).miR-222-3p inhibitor could reverse the effect of low GAS5 expression of hPDLSCs(P<0.05).The results implies that expression of GAS5 is up-regulated after osteogenic differentiation of hPDLSCs,and down-regulation of GAS5 expression can inhibit the osteogenic differentiation of hPDLSCs by targeting miR-222-3p. |
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| Keywords: | Periodontal ligament stem cells GAS5 Osteogenic differentiation miR-222-3p |
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