髓过氧化物酶在重症急性胰腺炎相关性腹腔积液致急性肺损伤中的表达及意义

目的探讨髓过氧化物酶在重症急性胰腺炎相关性腹腔积液致急性肺损伤中的表达及意义。方法成年wistar大鼠45只，随机分为3组：①阴性对照组（C组）：开腹后仅翻动胰腺组织；②重症急性胰腺炎组（S组）：3．5％牛磺胆酸钠胰胆管逆行注射制造重症急性胰腺炎模型；③腹腔注射组（E组）：取S组大鼠胰腺匀浆及腹腔积液，于正常大鼠腹腔内注射。于造模后3h、6h、12h分批处死大鼠，采下腔静脉血2ml行淀粉酶测定；取左肺下叶组织行MPO检测；胰腺及肺组织进行病理评分。结果S组及E组肺泡间质水肿、出血，并有中性粒细胞、巨噬细胞浸润。在不同时相，S组和E组血清胰淀粉酶、肺髓过氧化物酶均高于C组（P〈0．01），E组升高的程度小于S组。结论重症急性胰腺炎相关性腹腔积液可以导致急性肺损伤，肺组织中髓过氧化物酶表达升高，提示其参与了急性肺损伤的发生过程，其原因可能与相关性腹腔积液激活了粒细胞有关。

The Expression and Significance of Myeloperoxidase in Acute Lungs Injury of Severe Acute Pancreatitis Associated Ascetic Fluid

ObjectiveTo investigate the expression and significance of myeloperoxidase(MPO) in acute lungs injury of severe acute pancreatitis associated ascetic fluid. MethodsForty-five adult wistar rats were randomly assigned into the group of negative control (group C, n=15), the group of severe acute pancreatitis (group S, n=15) and the group of peritoneal injection (group E, n=15). The group C was cut peritoneum and flipped pancreases softly. In group S, 3.5% sodium taurocholate was injected retrograde in pancreatic and bile duct to establish the model of severe acute pancreatitis, and the pancreatic homogenate and ascites of the group S was injected into abdominal cavity of group E rats. After animal model established, rats were killed at 3h, 6h and 12h point. The blood of inferior vena cava was sucked for determination of amylase.The inferior lobe of left lung was cut for myeloperoxidase detection.And pathology was regularly done about pancreas and lungs. ResultsInterstitial edema, hemorrhage and infiltration of neutrophilic granulocyte and macrophage were observed in group S and E. At different time point, the amylase levels of blood and myeloperoxidase of lungs in group S and E were significantly higher than those of group C, and the increasing degree of group E was smaller than group S. ConclusionAcute lung injury can be induced by the severe acute pancreatitis associated ascetic fluid. The expression of myeloperoxidase of lungs was increased to induce acute lungs injury.The reason may be concerned with activation of granulocyte by severe acute pancreatitis associated ascetic fluid.