贾第虫纯培养的建立

从腹泻患儿新鲜粪便内,浓集、纯化贾第虫包囊。用生理盐水制成悬液,感染长爪沙鼠(Meriones unguiculatus)乳鼠。接种后第8d将受染鼠处死。用无菌术从上段小肠分离滋养体,接种于改良TYI-S-33培养基内,于37℃培养。培养后第14d,生长旺盛的虫体在培养管壁内面形成密集的细胞单层。生长高峰期和倍增时间分别在培养后的第120和15±2h。各批虫体经液氮冷冻复苏后,虫体复活率为53～80％。复苏虫体生长良好。本纯培养已维持9月余,传代100多次。

SUCCESSFUL AXEINIC CULTIVATION OF A LOCAL HUMAN STRAIN OF GIARDIA LAMBLIA IN SUCKLING GERBIL

G. lamblia cysts isolated from the fresh feces of a Giardia-infected boy in Beijing rural area were inoculated into suckling gerbils (Meriones unguiculatus). Trophozoites of G. lamblia obtained from the intestines of infected gerbils were cultivated in modified TYI-S-33 medium enriched with dehydrated bovine bile. The parasites grew luxuriantly and formed an intensive monolayer on the surface of the culture tube on day 14 after initial cultivation. The culture has been maintained for more than 12 months and more than 120 subcultures have been made. The growth curve of the organism showed that the peak growth of the trophozoites was attained at the 120th hour after seeding. The generation time was 15 +/- 2.0 hours. Periodic examinations of Giardia cultures for bacteria contamination, with Petri dishes of blood agar and beef broth, proved negative. After being cryopreserved in liquid nitrogen for 1 week or longer, the average viable rate of the organism was 65.7% and the resuscitated parasites grew luxuriantly in subcultures.