| 芦笋多糖对S180小鼠红细胞膜组分及流动性的影响 |
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| 引用本文： | 汲晨锋,季宇彬.芦笋多糖对S180小鼠红细胞膜组分及流动性的影响[J].天津中医药,2009,26(6):479-482. |
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| 作者姓名： | 汲晨锋 季宇彬 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100102;国家教育部抗肿瘤天然药物工程研究中心,哈尔滨,150076;哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨,150076
2. 国家教育部抗肿瘤天然药物工程研究中心,哈尔滨,150076;哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨,150076;哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨,150076 |
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| 基金项目： | 国家自然科学基金项目，黑龙江省自然科学基金项目，黑龙江省高校骨干教师项目 |
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| 摘 要： | 目的]考察芦笋多糖对S180小鼠红细胞膜组分及流动性的影响.方法]芦笋多糖对S180小鼠腹腔注射给药7d,应用聚丙烯酰胺凝胶电泳测定小鼠红细胞膜带3蛋白及血型糖蛋白A的相对含量,采用试剂盒测定红细胞膜磷脂、胆固醇和唾液酸含量,荧光偏振法结合探针1,6一二苯-1、3、5己三烯测定红细胞膜流动性.结果]芦笋多糖能增加S180小鼠红细胞膜表面带3蛋白、血型糖蛋白A的含量;升高S180小鼠红细胞膜磷脂含量,降低胆固醇含量;增加S180小鼠红细胞膜表面唾液酸含量;提高S180小鼠红细胞膜流动性.结论]芦笋多糖可以改善S180小鼠红细胞膜组分的异常变化,从而保持细胞膜的流动性,恢复红细胞的结构和功能.
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| 关 键 词： | 芦笋多糖 红细胞膜 细胞膜流动性 |
| 收稿时间： | 2009/9/16 0:00:00 |
Effect of asparagus polysaccharide on components and floating characteristics of erythrocyte membrane in S180 mice |
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| JI Chen-feng.Effect of asparagus polysaccharide on components and floating characteristics of erythrocyte membrane in S180 mice[J].Tianjin Journal of Traditional Chin Medicine,2009,26(6):479-482. |
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| Authors: | JI Chen-feng |
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| Institution: | School of Materia Mediea, Beijing University Of TCM, Beijing 100102, China;Engineering Reseach Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China;Center of Research on Life Sciences and Environmental Sciences, Harbin 150076, China;Engineering Reseach Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China;Center of Research on Life Sciences and Environmental Sciences, Harbin 150076, China;Institute of Materia Medica and Postdoctoral Program of Harbin University of Commerce, Harbin 150076, China |
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| Abstract: | Objective] To study the effect of asparagus polysaccharide on membrane components and floating characteristics of erythrocyte in S180 mice. Methods] S180 mice were administrated with asparagus polysaccharide i.p. for 7 days and we determined band 3 protein and glycophorin a content by SDS-PAGE, membrane phspholipid, cholesterol and sialic acid with test kit, the floating characteristics of membrane with fluorescence polariscopy combined with DPH. Results] It was showed that asparagus polysaccharide could increase band 3 protein and glycophorin a content on erythrocyte membrane, increased phospholipids content and decreased cholesterol content, in creased sialic acid content, adjust membrane floating characteristics. Conclusion] Asparagus polysaccharide can adjust the abnormality of S180 mice, and improve membrane floating characteristics, restore erythrocyte membrane structure and function. |
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| Keywords: | asparagus polysaccharide erythrocyte membrane floating characteristics |
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