胚胎干细胞的体外诱导分化模型

目的胚胎干细胞是具有全能性及无限制自我更新与分化能力的一类特殊细胞群体,通过对胚胎于细胞诱导分化研究的回顾和总结,期望能给胚胎干细胞的临床应用提供理论参考.资料来源应用计算机检索Highwirepress以及Medline数据库2000/2004期间的相关文章,检索词为"Embryonic stemcell,differentiation,induce",同时计算机检索万方数据库2000/2004期间的相关文章,检索词为"胚胎干细胞",限定文章类型为中文.资料选择对资料进行初审,选取与诱导分化有关的文章,纳入标准为研究原著.资料提炼选取与诱导分化有关的文章共369篇(其中外文364篇).通过阅读相关文献的摘要,从中选取15篇较全面介绍胚胎干细胞的诱导分化以及应用的文章.其中,全面介绍胚胎干细胞各种细胞模型,神经细胞模型,心肌细胞模型,上皮细胞模型,造血细胞模型.资料综合7篇文献较为全面的介绍胚胎干细胞可以诱导分化为神经细胞、心肌细胞、上皮细胞以及造血细胞等细胞模型,从以上文献以及单独介绍各种细胞模型的文章中分别对各种细胞模型的研究概况进行归纳整理.结论胚胎干细胞经过定向诱导分化后可获得神经细胞、心肌细胞、上皮细胞以及造血细胞等细胞模型,这些模型可以用于细胞发育分化的分子机制,并对药物筛选和临床细胞治疗提供良好应用前景.

Induced differentiation model of embryonic stem cell in vitro

OBJECTIVE: Embryonic stem cells (ES cells) is a special kind of cell population with totipotency and uninhibited self-renewal property. Researches on induced differentiation of ES cells were received and summed up to provide reasonable and constructive suggestions for clinical application of ES cells.DATA SOURCE: Papers published in Highwire press and Medline database were retrieved with keywords "Embryonic stem cell, differentiation, and induce" from 2000 to 2004. Additionally, papers published in Wanfang database were also retrieved with keywords "Embryonic stem cell" in Chinese from 2000 to 2004.STUDY SELECTION: Data were analyzed firstly in order to select papers related to induced differentiation. The inclusion criteria were selection of the original works, but the summaries and Meta analysis were excluded.DATA EXTRACTION: A total of 369 papers were chosen according to correlation with keywords; among them, 364 papers were in foreign language. After reading through summaries of these papers, 15 papers thoroughly discussing induced differentiation of ES cells were chosen for further intensive reading. Papers discussed various kinds of differential models, neural cells, cardiac muscle cells, epithelial cells and hematopoietic cells.DATA SYNTHESIS: The induced differential models of neural cells, cardiac muscle cells, epithelial cells and hematopoietic cells from ES cells were introduced comprehensively in the seven literatures. Based on these references mentioned above and other references which introduced cell model separately, all data were generally studied, sorted out and summed up.CONCLUSION: ES cells can be induced to be various kinds of cell models such as neural cells, epithelial cells, cardiac muscle cells and hematopoietic cells, which may be used to clarify the mechanism of cellular development and differentiation to provide good respects for clinical cellular therapy and screening of medicines.