氟化钠对乳鼠成骨细胞c—fos,c—jun基因表达及细胞增殖？…

目的 探讨氟对钠（ＮａＦ）对离体成骨细胞ｃ－Ｆｏｓ、ｃ－Ｊｕｎ基因表达的调控及细胞增殖的影响。方法 采用新生大鼠头盖骨分离成骨细胞,在不同浓度ＮａＦ（１０＾－５ｍｏｌ／Ｌ、１０＾－４ｍｏｌ／Ｌ、１０＾－３ｍｏｌ／Ｌ）中进行培养,用四甲基偶氮唑蓝比色法检测细胞增殖,用免疫组化ＳＰ法结合计算机图像处理系统检测ＮａＦ诱导的ｃ－Ｆｏｓ、ｃ－Ｊｕｎ表达。结果 实验表明ＮａＦ可刺激成骨细胞增殖,并诱导成骨细胞

Effects of fluoride on the expression of c-fos and c-jun genes and cell proliferation of rat osteoblasts

Objective To investigate the effects of sodium fluoride (NaF) on the expression of c Fos and c Jun genes and osteoblast cell proliferation of rat osteoblasts. Methods Osteoblastic cells were isolated from baby rat calvaria, and cultured in the presence of different doses of NaF (10 -5 mol/L, 10 -4 mol/L, and 10 -3 mol/L). Cell proliferation was measured by the MTT method, and c Fos and c Jun expression was detected by the immunohistochemistry combined with image analysis compute system. Results Results from the MTT assay showed that NaF increased the proliferation of rat osteoblast and induced the expression of c Fos and c Jun genes. The increases of c Fos and c Jun expression by the 3 different doses of NaF were 5 4%, 15 4% and 42 3% for the c Fos gene and 12 1%, 14 4% and 38 6% for c Jun gene respectively ( P< 0 05 and P< 0 01). Conclusion NaF increased rat osteoblastic cell proliferation and the expression of genes involved in cell proliferation.