CYP1A1基因多态性和GSTM1缺失与肺癌易感性的关系

[目的]探讨CYPlAl基因异亮氨酸(Ile)-缬氨酸(Val)位点多态性和GSTMl缺失与肺癌易感性的关系。[方法]以病例-对照方法,采用PCR技术检测82例原发性肺癌患者和91例对照者的CYPlAl基因Ile-Val位点多态性与GSTMl基因的缺失。[结果]Ile-Val3种多态基因型在肺癌组和对照组分布差异有显著性(P<0.05),Ile／Val、Val／Val基因型在肺癌组的分布频率明显高于对照组;logistic回归分析结果显示Ile／Val、Val／Val基因型患肺癌的危险性分别是Ile／Ile基因型的1.969(95％CI:1.012-3.828)倍和3.150倍(95％CI:1.278-7.761);GSTMl基因缺失在两组的分布频率差异有显著性(P<0.05,OR=2.157)。进一步联合CYPlAl多态性分析显示GSTMl缺失的个体同时携带Ile／Val或Val／Val基因型患肺癌的危险性较单独具有一种危险因子患肺癌的危险性显著增加(OR=5.538)。[结论]CYPlAl第7外显子的Ile／Val、Val／Val基因型和GSTMl缺失与肺癌的易感性有关,可望作为肺癌易感人群筛选的重要指标。

A Study on the Relationship between Polymorphism of CYP1A1, Lack of GSTM1 and Susceptibility to Lung Cancer

[Objective] To investigate the relationship between lung cancer susceptibility and polymorphisms of CYP1A1 gene Ile-Val and deletions of GSTM1.[Methods] Ile-Val polymorphisms of CYP1A1 and the deletion of GSTM1 were detected with the methods of ASA-PCR and PCR-RFLP in 82 cases of lung cancer group and 91 cases of control group.[Results] The frequencies of 7th exon polymorphisms in case group and control group were statistically significantly different(P<0.05),the distribution value of Ile/Val and Val/Val genotypes in lung cancer group is significantly higher than that in control group.Logistic regression analysis showed that the risk of Ile/Val, Val/Val to lung cancer were 1.969(95% CI: 1.278-7.761)and3.150(95%CI; 1.278-7.761)times respectively to Iie/Ile genotype.The deletion of CSTM1 in case group was higher than in control group(P<0.05,OR=2.157).However,when combined with polymorphisms of CYP1A1,the result showed that the individuals with GSTM1 deletion and Ile/Val or Val/Val genotype have a higher risk to develop lung cancer than others.[Conclusion]The results suggest that Ile/Val and Val/Val genotypes and CSTMl deletion are related to the susceptibility to lung cancer in smoking subjects, they are maybe one of the risk factors of lung cancer.